神经生长因子对2,5-己二酮致运动神经元线粒体功能损伤的影响

Effect of nerve growth factor on mitochondrion dysfunction of motor neuron induced by 2,5-hexanedione

目的探讨神经生长因子(NGF)对2,5-己二酮所致运动神经元细胞线粒体功能损伤的影响。方法用10mmol/L和20mmol/L2,5-己二酮给运动神经元VSC4.1细胞处理12h后,检测VSC4.1细胞活性,NGF的蛋白表达以及线粒体膜电位的变化;20mmol/L2,5-己二酮与VSC4.1细胞作用12h后,去除2,5-己二酮加入不同浓度的NGF,检测细胞活性和线粒体膜电位的变化。结果10mmol/L和20mmol/L2,5-己二酮与运动运动神经元VSC4.1细胞共同培养12h后,细胞活性分别下降了17%和23%;VSC4.1细胞内NGF的表达分别下降了33.5%和34%;线粒体膜电位由39.36分别下降到29.57和23.80。VSC4.1细胞与2,5-己二酮作用12h后用50ng/ml和100ng/mlNGF处理,细胞的活性增加了6.38%和23.40%;细胞线粒体膜电位也由26.03增加到30.18和31.50(P<0.05)。结论NGF可以通过改善线粒体膜电位来修复2,5-己二酮导致的运动神经元线粒体功能损伤。

To explore the effect of nerve growth factor（NGF） on mitochondrion dysfunction of motor neuron induced by 2,5-hexanedione（2,5-HD）. Methods VSC4.1 cells （a cell line from motor neuron） were incubated 2,5-HD at the doses of 10mmol/L and 20mmol/L. VSC4.1 were incubated with 20mmol/L 2,5-HD for 12 hours and then 2,5-HD and serum were removed and same cells were cultured with NGF-contained medium. The cell viability was measured by MTr method. The mitochondrlon membrane potential （△∮m） was detected by Rho-123 and expression of NGF protein was detected by immunofluorescence and flow cytometry. Results VSC4.1 cells were treated with 2,5-HD at doses of 10mmol/L and 20mmol/L for 12 hours, when compared with the control group, the VSC4.1 cell viabilities decreased 17% and 23%, and expressions of NGF of VSC4.1 cells also decreased（33.5%, 34%）, and levels of mitochondrion membrane potential （△∮m） of VSC4.1 cells decreased from 39.36 to 29.57 and 23.80, respectively. 50 and 100ng/ml NGF were added into the culture medium when VSC4.1 cells were treated with 2,5-HD at the doses of 20mmol/L for 12h. The viabilities increased 6.38% and 23.40%. Mitochondria membrane potential of VSC4.1 cells increased（ from 26.03 to 30.18 and 31.50）. Conclusion The improvement of the mitochondrion dysfunction induced by 2,5-HD could be associated the mitochondrion membrane potential （△∮m） increased by NGF.