市售腊肠中总亚硝基化合物检测

Determination of total N-nitroso compounds in sausage on the market

目的 建立腊肠总亚硝基化合物（TNOC）测定方法，调查腊肠中TNOC污染情况。方法 在以往液体标本测定方法的基础上建立腊肠TNOC的化学裂解-热能分析仪测定方法，该法具有区分热和酸不稳定物质的优点。随机采集市售腊肠32份，采用化学裂解-热能分析仪法测定腊肠中总亚硝基化合物含量。结果 研究发现，AG1-X8阴离子树脂可以有效去除样品中硝酸盐的干扰，又不影响亚硝基化合物的测定；0.2％HCl／HAc既能很好裂解NaNO2，又不会使不稳定的亚硝酰胺大量分解；样品加标平均回收率为86％（83％～89％）；TNOC检出限为6.6μg（N-NO）／kg，相对标准差为5.9％。32份腊肠样品中’TNOC检出率为93.8％；平均含量为49.09μg／kg（CI 39.15～126.35μg/kg）。结论 该方法具有准确性和可重复性。腊肠中TNOC污染较普遍，应当加强这方面的食品卫生监督。

To survey the polluted level of total N-nitroso compounds（TNOC） in sausage on the market by the method established for the determination of TNOC in the sausage. Methods The method of chemical fission-thermal energy analyzer was based on the previous method for the determination of TNOC in biological fluids which could distinguish between thermo-labile compounds and acid-labile compounds （TAC）. Thirty-two samples of sausage were selected by a random sample procedure on the market. Chemical fission-thermal energy analyzer was used in the analysis of TNOC in the samples.Results The results showed that nitrate from the sample was effectively removed by AG1-X8 anion exchange resin, and did not affected detection of N-nitroso compounds（NOC）. 0.2 % HCI/HAc was optimum concentration for TAC, and did not decompose unstable nitrosamides, Spiked with NOC mixture, the recovery in the sausage was 86% （83 % - 89% ）. The detection limit of this method was 6.6μg（ N-NO）/kg with 5.9% the relative standard deviation. TNOC detection rate and the average levels in the sausage were 93,8% and 49.09μg/kg（95% CI 39.15 - 126.35μg/kg）, respectively. Conclusion This method may be veracity and could be repeated and could use for determination of TNOC in sausage.