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酶联免疫吸附试验在微囊藻毒素检测中的应用进展 被引量:12

Application of ELISA for microcystins detection
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摘要 微囊藻毒素(MCs)是蓝绿藻属产生的一类密切相关的环状七肽毒素,其毒性主要表现在特异性抑制细胞内的蛋白磷酸酶(分别为PP1和PP2A)。目前有关微囊藻毒素分析检测的研究方法有高效液相色谱(HPLC)、质谱(LC-MS)、蛋白磷酸酶抑制试验(PPIA)和酶联免疫吸附试验(ELISA)等,本文主要介绍了应用酶联免疫吸附法检测MCs的研究进展,并对ELISA在天然水中检测微囊藻毒素的应用前景进行了展望。 Micmcystins (MCs) may be group of closely related toxic cyclic heptapeptides produced by common cyanobacteria( blue-green algae). Their toxicity could be associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). There are some methods for analyzing and detecting MCs in aquatic environment, such as HPLC, LC-MS, PPIA, and ELISA. This review introduces advances on MCs determined by enzyme-linked immunosorbent assay (ELISA), and estimates the prospective development of ELISA for monitoring MCs in natural waters.
出处 《卫生研究》 CAS CSCD 北大核心 2007年第3期388-390,共3页 Journal of Hygiene Research
基金 广东省科技攻关(No.2006B40101013)
关键词 蓝藻水华 微囊藻毒素 单克隆抗体 酶联免疫吸附试验 cyanobactefial blooms microcystins monoclonal antibodies ELISA
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