镉诱导金属硫蛋白在华溪蟹组织中的表达

Metallothionein in Different Tissues of Freshwater Crab Exposed to Cadmium

采用体外暴露染毒法,研究了不同浓度与时间条件下,镉诱导河南华溪蟹(Sinopotamon henanense)金属硫蛋白(metallothionein,MT)在肝胰腺、肌肉、鳃和卵巢中的表达差异。镉浓度分别为0、14·5mg/L、29mg/L和58mg/L;处理时间依次为1d、3d和5d。利用镉血红蛋白饱和法和火焰原子吸收分光光度法(AAS)测定MT的蛋白含量。结果显示,用不同的染毒浓度和处理时间,镉在组织中诱导产生MT的含量有较大差异,其中肝胰腺MT的诱导量最大,变化规律也最明显;肌肉中也有较大量MT的表达;而鳃和卵巢MT的诱导量均较低。此外,本文分析了镉的浓度与时间梯度对诱导MT表达的影响与毒性效应机制。结论:组织不同,染毒浓度及时间不同,镉诱导MT的表达也不同,具有一定的组织差异性和规律性。

In order to investigate the impact of waterborne cadmium on the content of metallothionein （MT） in hepatopancreas, muscle, gill and ovary of Freshwater Crab Sinopotamon henanense, the individuals with similar size and weight were exposed to different concentrations of cadmium （0, 14.5, 29 and 58 mg/L） for a duration of 1,3 or 5 days. The content of MT in the four tissues was measured by Cd-Hb Saturation and AAS. The results showed that there was a great variation in MT expressed in the tissues of S. henanense after exposing the crabs to different concentrations of cadmium and for different times. The MT content in hepatopancreas was the highest in the four tissues, and the MT expressed in the muscle was more than that in the gill and ovary. This research indicates that the content of MT may be affected by cadmium exposure and that MT contents are different in various tissues after cadmium exposure.