刺五加中紫丁香苷的制备及其含量测定

Study on Isolation Syringin and Determination the Concentration of Syringin in Acanthopanax Senticosus Harms

目的：首先对刺五加中有效成分紫丁香苷进行了提取分离和结构鉴定,自制定量用的紫丁香苷标准品。方法：建立了刺五加中紫丁香苷的反相高效液相色谱定量法：以甲醇-乙腈-水-冰醋酸（14∶1∶85∶1,v∶v∶v∶v）为流动相,检测波长270nm。结果：紫丁香苷在1.24~31.0μg/mL质量浓度内线性关系良好（r=0.9997）,平均回收率为100.1%（RSD 2.2%）。结论：该法简便快速,结果准确可靠。该方法可作为刺五加质量控制的手段之一。

Objective： Syringin which is the effective constituent of Acanthopanax senticosus Harms was isolated and identified. The quantity Reference of Syringin is made by ourself. Method ：A method for the determination of the concentration of syringin in Acanthopanax senticosus Harms was established by using HPLC. The mobile phase consisted of methanol - acetonitrile - water - glacial acetic acid （ 14： 1： 85：1 ,v： v： v： v） . The UV detection wavelength was set at 270 nm. Result： The calibration curve was linear in the range from 1.24 g mL^-l to 31.0 g mL^-1 ,with r = 0. 9997. The average recovery was 100.1% （ RSD2. 2% ）. Conclusions： The assay methods are simple ,rapid and with good reproducbility. This method can be used for quality study of Acanthopanax senticosus Harms.