摘要
目的 建立羚黄氨咖敏片中马来酸氯苯那敏、对乙酰氨基酚、咖啡因的高效液相色谱测定方法.方法 采用Hypersil-ODS柱,甲醇-0.05 mol/L磷酸二氢钾溶液(25:75,0.5%磷酸调节pH 3.0)为流动相,流速1.0 mg/min,检测波长216 nm.结果 马来酸氯苯那敏线性范围1.20~2.80 mg/L,平均回收率为97.90%,RSD为1.52%;对乙酰氨基酚150.00~350.00 mg/L,平均回收率为100.21%,RSD为0.92%;咖啡因9.00~21.00 mg/L,平均回收率为101.46%,RSD为0.90%.结论 本方法简便、结果准确,可用于制剂的含量测定和质量控制.
Objective To establish a methods of HPLC (high performance liquid chromatography) to the determination of chlorpheniramine maleate, paracetamol and caffeine in Linghuang'anjiamin tablets. Methods Hypersil-ODS column mobile phase composed of methanol and 0.05 mol/L of KH2PO4 (25:75, adjusted to pH3.0 with 0.5% pf H3PO4 ) was adopted. The flow rate was 1.0 ml/min. The UV detection wavelength was 216 nm. Results The standard curve of chlorpheniramine maleate was hnear over a range of 1.20 -2.80 mg/L. The average recovery was 97.90% with a RSD of 1.52%. The standard curve of paracetamol was linear over a range of 150.00 -350.00 mg/L. The average recovery was 100.21% with a RSD of 0.92%. The standard curve of caffeine was linear over a ranger of 9.00 - 21.00 mg/L. The average recovery was 101.46% with a RSD of 0.90%. Conclusion This method of HPLC is found to be simple and accurate, and it could be used for the analysis of the agent concentration and for the quality control.
出处
《临床军医杂志》
CAS
2007年第3期440-442,共3页
Clinical Journal of Medical Officers
关键词
羚黄氨咖敏片
马来酸氯苯那敏
对乙酰氨基酚
咖啡因
高效液相色谱
linghuang' anjiamin tablet
chlorpheniramine maleate
paracetamol
caffeine
high performance liquid chromatography