组织芯片技术及其在新药毒理学研究中的应用

Tissue microarray and its application in toxicologic research of new drugs

组织芯片(tissue microarray,TMA)也称组织微阵列,是将数十个甚至上千个微小组织标本整齐排列在一张载玻片上制成的高通量微阵列,是基因芯片的延伸和发展,可以同时进行多个标本的同一个指标的研究,具有体积小、耗材少、快速、含生物学信息量大等优点,该技术还可以与免疫组化(immunohistochemistry,ICH)、荧光核酸原位杂交(fluorescence in situ hybridization,FISH)、RNA原位杂交(RNA in situ hybridization,RNA-ISH)等技术相结合,在基因、转录和表达产物的不同表达分子水平进行研究。本文结合组织芯片技术的特点,对其在新药毒理学研究中的应用进行初步探讨。

Tissue microarray is a high-throughput microarray and the extendation and development of gene biochip allowing tens up to thousands of tissue sample on a single glass slid. Tissue microarray technology can do the simultaneous analysis of multi-samples and contain highly quantity of biology information with a small-bulk, economic, and speedy manner. It also can be integrated with immunohistochemistry, fluorescence in situ hybridization, and RNA in situ hybridization, doing analysis on the different molecule levels of gene, transcription, and expression. The characteristic of tissue microarray and its application in the toxicological research of new drugs were introduced.