TGF-β1mRNA在胃癌中表达及其与淋巴转移、生存率的相关性研究

Study of the correlation between the expression of TGF-β1mRNA and lymphatic metastases,survival rate with gastric cancer

目的:研究转化生长因子-β1mRNA(TGF-β1mRNA)在胃癌组织中的表达及其与淋巴结转移、术后生存率的关系。方法:用原位杂交法检测66例胃癌切除标本中癌组织和10例癌周组织TGF-β1mRNA表达;结合临床资料分析TGF-β1mRNA与胃癌患者术后生存率的关系。结果:胃癌患者中66.67%在术后5年内死亡。胃癌中TGF-β1mRNA阳性表达率86.36%。TGF-β1mRNA表达阴性或弱阳性者47.50%术后生存5年以上,52.50%5年内死亡,TGF-β1mRNA表达呈中、强阳性者11.54%术后生存5年以上,88.46%5年内死亡(P<0.05)。无淋巴结转移者TGF-β1mRNA阳性表达率75.00%,有淋巴结转移者TGF-β1mRNA阳性表达率97.06%(P<0.05)。TGF-β1mRNA高表达与胃癌的进展呈正相关。结论:TGF-β1可促进胃癌生长,增加胃癌的侵袭能力和淋巴转移。提示对胃癌患者行TGF-β1mRNA检测,可判断胃癌的进展和预后。

Objective：To study the correlation between the expression of TGF-β1mRNA in gastric cancer tissues and the tumor lymph node metastasis and survival rate after operation ,the expression can be used to estimate progression and prognosis. Methods： Using in situ hybridization method,66 specimens and 10 normal tissue specimens （controls） were tested for the expression of TGF- β1mRNA. The survival rate after operation was investigated also. The correlation between TGF-β1mRNA and survival rate was analyzed. Results：The gastric cancer in 66 specimens died in 5 year was 88.46%（23/26）. The positive expression rate of TGF- β1mRNA in gastric cancer was 86.36%（57/66）,while it was 10.00%（1/10） in controls,indicating significant difference between them（P 〈 0.01 ）. The negative and lower positive expression of TGF-β1mRNA in those who died after 5 years was 47.50%（19/40） ,in those who died in 5 year was 52.50%（21/40）,indicating significant difference between them （P 〈 0.05）. The middle and strong positive expression of TGF-β1mRNA in those who died after 5 years was 11.54%（3/26）, in those who died in 5 year was 88.46% （23/26）. The positive expression of TGF-β1mRNA in those who have no tumor lymph node metastasis was 75.00%（24/32） ,while it was 97.06%（33/34） in those who have tumor lymph node metastasis, indicating significant difference between them （P 〈 0.05 ）. There was positive correlation between the higher expression of TGF-β1mRNA and the worse prognosis and rapid progression of gastric cancer. Conclusion：TGF-β1 can stimulate gastric cancer grow and become more aggressive and the tumor lymph metastasis. The detection of TGF-β1mRNA can be used to estimate gastric cancer patients,progression and prognosis.