摘要
用20个随机引物对7个不同来源的鲍氏层孔菌菌株进行了RAPD分析.结果表明:20个随机引物中,有17个引物的扩增产物DNA条带表现出明显的多态性,不同引物对供试菌株扩增出现的DNA条带数目少则10条,多达33条.DNA片段从250bp到2000bp;采用17个引物对7个鲍氏层孔菌菌株共扩增出DNA片段带377条,不同引物扩增出的DNA片段谱带存在较大差异.采用UPGMA系统聚类法,将7个菌株聚类为两大类,能直观准确地揭示菌株间的差异并加以鉴别.
In this paper, random amplified polymorphic DNA （RAPD） analysis was conducted with 20 random primers in 7 strains of Phellinus baumii collected from different localities. The results showed that 17 of the 20 random primers were polymorphic ones. The DNA bands derived from each primer amplifying in tested strains ranged from 10 to 33. The size of the amplified DNA fragments ranged from 250 to 2000 base pairs. Of each test primer, a wide variation in banding profiles was observed among the 7 strains of P. baumii. A total of 377 band positions were scored for all of the test strains, which differed significantly among the bands from different primers. UPGMA cluster analysis subdivided the test strains into two groups, which was helpful to find out the difference among the test strains and to distinguish them directly.
出处
《应用生态学报》
CAS
CSCD
北大核心
2007年第6期1316-1320,共5页
Chinese Journal of Applied Ecology
基金
吉林省教育厅资助项目(20036)
