维生素C对水中有机物致小鼠外周血淋巴细胞DNA损伤的抑制作用

Inhibitory Effect of Vitamin C on Mice Lymphocyte DNA Damage Induced by Organic Extracts from Water

背景与目的:探讨维生素C水溶液对水中有机物致外周血液淋巴细胞DNA的损伤是否具有抑制作用。材料与方法:固相萃取水中有机物,用不同浓度维生素C水溶液(50、100、200mg/kg)与有机物以不同方式处理(同时灌胃、维生素C预处理、维生素C后处理),分别给小鼠灌胃3d后,取外周血淋巴细胞进行单细胞凝胶电泳实验,检测细胞DNA的损伤情况。结果:维生素C(100mg/kg、200mg/kg)能不同程度地抑制小鼠外周血淋巴细胞DNA损伤;中、高剂量水中有机物(每kg体重灌胃量相当于16、80L水样中的有机提取物)对小鼠外周血DNA有明显的损伤作用,其尾DNA含量、尾矩、椭圆矩、尾面积均高于对照组,且差异均具有统计学意义(P<0.05或P<0.01);维生素C具有抑制水中有机物所致小鼠外周血淋巴细胞DNA损伤的作用,其中维生素C100mg/kg、200mg/kg组尾DNA含量、尾矩、椭圆矩、尾面积均高于对照组,且差异具有统计学意义(P<0.05或P<0.01)。结论:维生素C能够降低自发的细胞DNA损伤并对水中有机物致小鼠外周血淋巴细胞DNA的损伤具有抑制作用。

BACKGROUND ＆ AIM： To study the inhibitory effects of Vitamin C on lymphocyte DNA damage induced by organic extracts from water. MATERIALS AND METHODS：Solid phase extraction was used to extract the organic pollutants in tap water and the lymphocyte DNA damage in mice treated with Vitamin C （50,100,200 mg/kg） and organic extracts of water in several different ways （simultaneous treatment with Vitamin C and organic extracts of water, pre-treatment and post-treatment with Vitamin C） was studied. After 3d of gastric gavages, peripheral blood was taken from experimental mice to extract the lymphocytes. Then single cell gel electrophoresis test was used to detect the inhibition of DNA damage. RESULTS：Vit C （100 mg/kg,200 mg/kg） could inhibit the lymphocyte DNA damage in mice. Lymphocyte DNA in mice could be damaged by medium and intake high doses of organic extracts in water （about 16L and 80L water） , and the data of tail DNA, tail moment, olive moment and tail area were significantly higher than the control（P 〈 0.05 or P 〈 0.01） This lymphocyte DNA damage could be inhibited by Vit C to different degrees, and the data of tail DNA, tail moment, olive moment and tail area of Vit C（100 mg/ kg, 200 mg/ kg） were higher than the control and statistically different （P 〈 0.05 or P 〈 0.01）. CONCLUSION： The innate cell DNA damage could be inhibited by Vit C and Vit C could also inhibit the lymphocyte DNA damage in mice induced by organic extracts from water.