β_2-AR基因+491C/T多态性与新疆哈萨克族男性原发性高血压的关联研究

The Study Of the Association Between the +491C/T polymorphism of β_2-adrenergic Receptor Gene and Essential Hypertension in Kazakns of Xinjiang in male

目的：研究陡肾上腺素能受体（β2-AR）基因＋491C／F多态性与新疆男性哈萨克族人原发性高血压（Essential Hypertension EH）的关系。方法：以人群为基础的病例一对照研究，选择175例30岁．60岁男性哈萨克族人（83例EH者与92例血压正常者）为研究对象，酚／氯仿法提取外周血白细胞基因组DNA，应用PCR—RFLP技术，检测＋491C／T基因型，并观察各基因型、等位基因频率在该人群中分布及其差异。结果：只检测出两种基因型CC、CT，分布频率为9r7．71％、2．29％，等位基因C，T频率为98．86％、1．14％。CC、CT在EH组分布频率分别为95．18％、4．82％，正常血压组中未检出CT基因型。经Fisher’s精确概率检验，两组基因型、等位基因分布频率差异均有统计学意义；基因型间收缩压差异有统计学意义。结论：β2-AR基因＋491C／T多态性与新疆男性哈萨克族人EH尤其是高收缩压相关联，提示该多态性可能是男性哈萨克族人EH的易感因素。

Objective： To study the molecular pathogenic mechanism of predispose to hypertension in Kazakns, we investigated the association between the ＋ 491C/T polymorphism of β2- AR gene and essential hypertension in Kazakns of Xinjiang in male. Methods：In this population- based ease- control study, we recruited 175 Kazakns subjects （83 hypertensives and 92 normotensives ,30- 60 years old） from the pastoral areas in Xinjiang . Peripheral blood were collected from all recruited subjects. The DNA was extracted by means of a standant phenol- chloroform method. Then the genotypes of β2 - All was identified by PCR - restriction fragment length polymorphism in all the subjects. The distribution of genotypic and allelic frequencies between hypertensives and normotensives were studied. Results： In the population, the frequencies of the genotype CC, CT were 97.71% and2.29% respectively, no IT was found, the genotypic and allelic frequencies were in Hardy - Weinberg equilibrium（Χ^2 = 0.023, P = 0. 878）. These two genotypic frequencies were 95.18% and 4.82% respectively in essential hypertensives, while no CT in normotensives. The allelic frequencies of C,T were 97.59% ,2.41% and 100% ,0% in two groups and there is a significant differences between these two groupso In addition, systolic blood pressure has significant difference in the groups classified according to genotypes. Conclusion： The ＋ 491C/T polymorphism of β2 - All gene may be associated with essential hypertension in male Kazakns, especially in systolic blood pressure . which implicates that it is possible one of the susceptible factors of essential hypertension in Kazakns of Xinjiang.