摘要
轮状病毒、诺瓦克病毒和星状病毒是引起病毒性胃肠炎的主要病原因子。研究采用JV12/JV13、P1/P2和Mon340/Mon348三对引物,建立了同时检测这3种病毒的多重RT-PCR技术,并应用于128份临床粪便样本的检测,检出轮状病毒62份(48.44%),诺瓦克病毒8份(6.25%),星状病毒11份(8.59%)。在灵敏度试验中,轮状病毒的检测灵敏度为5pg/mL、诺瓦克病毒和星状病毒的检测灵敏度均为50pg/mL。该研究所建立3种常见胃肠炎病毒的多重RT-PCR方法具有特异性强、灵敏度高的特点,可用于临床病原诊断和溯源。
Rotavirus, norwalk virus and astrovirus are the major causes of viral gastroenteritis. Primers JV12/JV13, P1/P2 and Mon340/Mon348 were used to develop a multiplex RT-PCR system to simultaneously detect norwalk virus, rotavirus and astrovirus in clinical fecal samples. In a total of 128 samples, 62 was rotaviras, 8 was norwalk virus and ll was astrovirus, The detection limits was 5pg/mL for rotavirus, 50pg/mL for norwalk virus and astrovirus respectively, The multiplex RT-PCR system can be used to detect norwalk virus, rotavirus and astrovirus for routine monitoring and risk assessment in diseases outbreak with high specificity and sensitivity.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第3期401-405,共5页
Microbiology China
基金
广东省重大科技攻关项目(No.2002B3100103)
