药用真菌桑黄液体深层发酵条件的优化

Optimum of Submerged Fermentation Parameters for Medicinal Fungus Phellinus baumii

以菌丝体生物量和多糖产量为主要指标,对桑黄(鲍氏层孔菌)的深层发酵条件进行了优化,通过单因素试验和四因素三水平正交试验筛选出了桑黄液体发酵的培养基,结果表明,最适培养基为:葡萄糖5g/L,玉米粉40g/L,豆饼粉20g/L,KH2PO41.5g/L,MgSO41g/L。进一步通过培养条件的优化,得到最适菌丝体生长的液体发酵条件为:培养温度28℃,摇床转速140r/min,pH值自然,接种量10%,装液量100mL(250mL三角瓶),发酵周期132h。

Taking the mycelial biomass and production of polysaccharide as detecting indices, the optimal submerged fermentation parameters for medicinal fungus Phellinus baumii were investigated by various media and conditions, including single factor test and L9 （3^4） orthogonal test. The optimal culture was ： glucose 5g/L, corn flour 40g/L, soybean cake powder 20g/L, KH2 PO4 1.5g/L, MgSO4 1g/L. The optimal fermented condition was ：culture temperature 28 %, rotation speed 140r/min, pH normal, inoculation volume 10%, the amouht of liquid 100mL（250mL canonical flask）, and fermentation time 132h.