儿科常见感染性疾病患儿血浆甘露糖结合凝集素浓度检测

Measurement of mannose-binding lectin plasma levels in children with different infectious diseases

目的比较几种儿科常见感染性疾病患儿血浆甘露糖结合凝集素(MBL)浓度。方法采用ELISA法检测血浆MBL浓度,组间比较采用SPSS软件11.0版的非参数检验。结果健康组(105例)、反复呼吸道感染组(57例)、急性呼吸道感染组(21例)、急性阑尾炎组(17例)、体表局部脓肿组(13例)的血浆MBL浓度中位数分别为1065、798、1477、1463和1707ng/ml,健康组MBL浓度低于急性呼吸道感染组(P=0.030)和体表局部脓肿组(P=0.021),但与反复呼吸道感染组(P=0.052)相比差异无统计学意义;反复呼吸道感染组与其他感染组相比,MBL浓度明显低于急性呼吸道感染组(P=0.011)和体表局部脓肿组(P=0.005)。结论低血浆MBL浓度可能是儿童反复呼吸道感染的影响因素之一。

Objectives To investigate the difference of mannose-binding lectin （MBL） plasma levels in children with different infectious diseases. Methods The patients were divided into four groups. And MBL plasma levels were measured by ELISA method with human MBL ELISA kit, and the differences of MBL plasma levels among different groups were analyzed with SPSS 11.0 statistic analysis software. Results The medians of MBL plasma levels in healthy group （105 cases）, recurrent respiratory tract infection （RTI） group （57 cases）, acute RTI group （21 cases）, acute appendicitis group （17 cases）, and localized abscess group （13 cases） were 1 065 ng/ml, 798 ng/ml, 1 477 ng/ml, 1 463 ng/ml and 1 707 ng/ml, respectively. In comparison with the healthy group, the MBL levels was significantly higher in acute RTI group （P = 0.030） and localized abscess group （P = 0.021 ）, with no significant difference among recurrent RTI group, acute RTI group, and localized abscess group （P = 0.052）. In comparison with the acute RTI group, localized abscess group, and tympanitis group, the MBL levels was significantly lower in recurrent RTI group （P = 0.011, P = 0.005, P = 0.041, respectively）. Conclusions Low MBL plasma levels was one of the important factors that cause recurrent RTI in children.