载脂蛋白B基因EcoRⅠ位点多态性对儿童生长发育及营养状况的影响

Effect of EcoR I locus polymorphism of apolipoprotein B gene on childhood's growth and nutritional status

目的探讨载脂蛋白B(ApoB)基因EcoRⅠ位点多态性对儿童生长发育及营养状况的影响。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术结合测序的方法确定70例健康体检儿童ApoB基因EcoRⅠ位点的基因型,同时计算体重指数(BMI)作为儿童生长发育的评价指标,检测血清总蛋白(TP)、白蛋白(AIB)、球蛋白(GLO)、白蛋白/球蛋白(A/G)、脂蛋白a(LPa)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、非高密度脂蛋白胆固醇(non-HDL)、低密度脂蛋白胆固醇(LDL-C)、ApoA1、ApoB及ApoA1/B作为营养状况的评价指标。结果①EcoRⅠ位点各基因型的频率分别为E+/E+72.9%、E+/E-18.6%、E-/E-8.6%,等位基因E+与E-的频率分别为82.1%和17.9%;②各基因型组间比较显示血清AIB、HDL-C、TP、Lpa、TC和ApoA1差异具有统计学意义;③E+/E-组血清TC、HDL-C、ApoA1水平显著高于E+/E+组(P<0.01);E-/E-组血清TP及AIB浓度显著低于E+/E+组(P<0.01);E-/E-组血清TP、AIB、Lpa和ApoA1水平显著低于E+/E-组;④等位基因E-组血清TP和AIB水平显著低于E+组(P<0.05、<0.01),而TC、HDL-C及LDL-C水平显著高于E+组(P<0.05);⑤血清LDL-C分别与TP及AIB呈高度负相关(P<0.05),而HDL-C与AIB呈显著正相关(P<0.05)。结论ApoB基因EcoRⅠ位点多态性对儿童生长发育不构成影响,但可以通过影响血脂代谢对儿童的营养状况产生效应。

Objectives To explore the effect of EcoR Ⅰ locus polymorphism of apolipoprotein B （ApoB） gene on childhood growth and nutritional status. Methods EcoR Ⅰ genotypes of ApoB gene in 70 healthy children were analyzed with polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method and gene sequencing. Body mass indexes （BMI） was evaluated as the indicator of growth. Total protein （TP） , albumin （AIB） , globulin （GLO） , A/G, lipoprotein a （ LPa）, total cholesterol （TC）, triglyceride （TG）, high-density lipoprotein cholesterol （ HDL-C）, non-HDL, low-density lipoprotein cholesterol （LDL-C）, ApoA1, ApoB, and ApoA1/B were detected as the evaluation indicators of nutritional status. Results （ 1 ） The distribution frequencies of the EcoR Ⅰ genotypes were 72.9% （E^＋/E^＋）, 18.6% （E^＋/E^-） , 8.6% （E^-/E^-）, respectively. The allele frequencies of E^＋ and E^- were 82.1% and 17.9% respectively. （2） The differences of serum AIB, HDL-C, TP, Lpa, TC and ApoA1 among 3 genotypes were significant. （3） The serum levels of TC, HDL-C, ApoA1 were higher in E^＋/E^- group than those in E^＋/E^＋ group （P 〈 0.01 ） . TP and AIB were lower in E^-/E^- group than those in E^＋/E^＋ group （P 〈 0.01 ） . TP, AIB, Lpa and ApoA1 were lower in E^-/E^- group than those in E^＋/E^- group. （4） The serum levels of TP and AIB were significantly lower in E^- group than those in E^＋ group （P 〈 0.05, 〈 0.01 ）, while TC, HDL-C, and LDL-C were higher in E^- group （P 〈 0.05）. （5） Negative correlations were found between LDL-C and TP, LDL-C and AIB （P 〈 0.05）, while there was a positive correlation between HDL-C and AIB （P 〈 0.05）. Conclusions The EcoR Ⅰ locus polymorphism of ApoB gene has no effect on childhood growth and development, but it can influence the nutritional status through adjusting the metabolism of serum lipids.