利用大孔吸附树脂对黄芩总黄酮的纯化研究

Studies on Purification of Flavones from Scutellaria Bacalensis Georgi by Adsorptive Resin

目的:比较5种大孔吸附树脂对黄芩总黄酮的吸附分离性能,筛选纯化黄芩总黄酮的最佳树脂。方法:采用紫外分光光度法测定黄芩总黄酮的含量,采用动态吸附法、静态吸附法及静态吸附动力学试验对5种大孔吸附树脂分离纯化黄芩总黄酮的性能进行对比。结果:AB-8、D101、SP850、HP20、SP825在静态吸附筛选试验中的黄酮吸附量分别为64.98%、73.12%、65.68%、74.39%、72.90%,在动态吸附筛选试验中,比吸附量分别为35.50、37.52、32.25、38.25、30.30mg.g-1,除SP850外,其它4种树脂吸附达到饱和所需时间均较短,均在5h左右。结论:综合考察解吸率、总黄酮回收率及醇洗物中总黄酮含量,分离纯化黄芩总黄酮以HP20树脂最为理想。

Objective ： To select the optimum adsorptive resin for purification of flavones by studying the purification and separation functions of different adsorptive resins. Methods ： UV spectrophotometry was used to analyze the content of flavones in Scutellaria Bacalensis Georgi. Static adsorption test. dynamic adsorption test and staticadsorptive dynamic test were used to contrast the function of separating and purifying the flavones from Scutellaria Bacalensis test, the adsorption 65.68%. 74. 39% Georgi by the five kinds of macroporous adsorption restins. Results ： In the static adsorption capacity of the AB -8, D101. SP850. HP20. SP825 types was 64. 98%. 73.12%. 72.90% respectively. And during the dynamic adsorptive test, the absorbiton ratio was 35.50. 37.52. 32.25. 30. 30mg·g^- 1 respectively. With the desorption rate, the total flavone recovery rate and the content of total flavone in the ethanol eluant were also used as the comprehensive evaluating criteria, we decided that the optimum resin for separating and purifying the flavones in Scutellaria Bacalensis Georgi was the HP20 type. The saturation times were short（ about 5 hours）except SP850. Conclusions： HP20 resin can be used to separate and purify the total flavones in Scutellaria Bacalensis Georgi.