高渗脱水药物对大鼠脑皮质优化散射系数的影响及其临床意义

Effects of hyperosmotic solution anhydration on reduced scattering coefficient in rat′s local cortex and clinical significance thereof

目的利用内置双光纤微创探头的近红外测量(NIRS)技术观察高渗脱水药物对大鼠脑皮质优化散射系数(μ′s)的影响。方法将SD大鼠随机分成3组:对照组(n=15)尾静脉推注0.9%氯化钠,甘露醇组(n=15)尾静脉推注20%甘露醇,7.5%氯化钠组(n=15)尾静脉推注7.5%氯化钠;剂量均为10 m l.kg-1。使用内置双光纤微创探头活体在位连续动态观察大鼠局部脑皮质μ′s的变化情况。结果对照组静脉推注0.9%氯化钠前后大鼠脑皮质μ′s未见明显变化,甘露醇组和7.5%氯化钠组μ′s分别下降了(2.67±0.35)%和(5.62±0.46)%;甘露醇组与7.5%氯化钠组与对照组之间、甘露醇组与7.5%氯化钠组之间差异均有统计学意义(P<0.05)。结论大鼠脑皮质局部μ′s可以作为高渗药物脱水作用的评估指标之一。

Objective To study the role of hyperosmotic solution anhydration on reduced scattering coefficient （μ＇s） in rats＇ local cortex and it＇s clinical significance. Methods Forty-five SD rats were divided into three groups randomly, which were treated with 0.9% NaCl（ control group, n = 15 ） ,20% mannitol （20% mannitol group, n = 15 ） and 7.5 % NaCl （7.5 % NaCl group, n = 15 ）through vena caudalis respectively. The change of μ＇s in every rat＇s local cortex was observed continuously by a bifurcated fiber probe in vivo in a mini-invasive way. Results No change of μ＇s was observed in the control group,while the μ＇s of the 20% mannitol group and 7.5% NaCl group decreased by （2.67 ± 0.35 ） % and （ 5.62 ± 0.46 ） % respectively. There were significant differences among the three groups（P 〈 0.05）. Conclusion The μ＇s in rat＇s local cortex observed can be applied in evaluating the anhydration induced by hyperosmotic solution.