原发性肝癌结肠癌缺失基因杂合缺失研究

Loss of heterozygosity of DCC genetic locus in primary hepatocellular cancer

目的：了解结肠癌缺失基因（ＤＣＣ基因）在原发肝癌发生发展中的作用。方法：采用多聚酶链延伸反应－限制性片段长度多态性分析（ＰＣＲ－ＲＦＬＰ）方法对３０例外科手术切除原发肝癌标本ＤＣＣ基因杂合缺失（ＬＯＨ）进行分析。结果：２８例信息个体中检出ＬＯＨ７例，占２５．０％。ＤＣＣ基因ＬＯＨ与临床病理参数和ＨＢＶ感染无显著相关。结论：ＤＣＣ基因ＬＯＨ可能参与了原发肝癌的发生发展。

Objective: To explore the role of the loss of heterozygosity (LOH) of DCC gene in the occurrence and development of primary hepatocellular cancer (HCC). Methods: LOH of DCC genetic locus was studied in 30 surgically resected specimens of HCC with PCR RFLP technique. Results: LOH was detected in 7 (25.0%) out of the 28 informative individuals. No significant correlation was found among LOH of DCC gene, clinical parameters and HBV infection. Conclusion: LOH of DCC gene may be involved in the occurrence and development of HCC.