摘要
以带顶芽的菊花茎段为外植体,接种在诱导侧芽生长培养基中（MS＋NAA0.2mg/L＋6-BA2.0mg/L）,25d左右诱导成芽,再将芽接种于愈伤组织诱导培养基中（MS＋NAA0.1mg/L＋6-BA3.0mg/L）进行继代培养,最后接种于生根培养基中（1/2MS十NAA0.1mg/L）,15d即可生根,30-35d平均生根率达100%。将生根试管苗开盖练苗,移栽,成活率达95%以上。
The stem segments of chrysanthemum with terminal buds were used as explants and inoculated into the medium （MS＋0. 2mg/L NAA＋2. 0 mg/L 6-BA） which could induce the growth of lateral buds. The explants were induced to be buds after 25 days. Then the buds were subcultured into the callus-inducing medium（MS＋0, lmg/LNAA＋3. 0rag/ L6-BA）. At last the callus were inoculated into the root-inducing medium（1/2MS＋0, lmg/L NAA）. The roots were induced in the following 15 days. The average rate of rooting was up to 100% during 30-35 days. The plantlets of chrysanthemum were domesticated in culture bottles without covers, then transplanted. The survival rate of them was above 95%.
出处
《北方园艺》
CAS
北大核心
2007年第6期208-210,共3页
Northern Horticulture
关键词
菊花
组织培养
快速繁殖
Chrysanthemum
Tissue culture
Rapid propagation
