光学免疫分析仪传感芯片的表面修饰及表征

Surface modifications of biochips for optical immuno-analytical equipment

光学免疫分析仪能够实现对环境污染物的现场快速检测,为了减小传感芯片对蛋白的非特异性吸附并实现对抗体的特异性反应,需要对其进行化学修饰。该文选取2,4-二氯苯氧乙酸(2,4-D)为目标物质,研究3种传感芯片修饰方案,并对修饰后芯片表面的物理形态、化学组成及其对蛋白的响应进行表征。实验结果表明,3种方案均有效地抑制了蛋白的非特异性吸附,且成功地实现了对2,4-D小分子配基的固定,其中双氨基聚乙二醇修饰的芯片基本不会吸附杂蛋白,而氨基葡聚糖修饰的芯片对2,4-D抗体具有更强的响应。因此,这3种修饰方案都适用于光学免疫分析仪传感芯片的制备。

Optical immunoanalytical equipment can be used for rapid detection of pollutants in the field. For rapid response, the biochip must be modified to minimize adsorption of the protein and to promote the specific reaction with the antibody. 2,4-dichlorophen- oxyacetic acid （2,4-D） was used as the test substance with three methods to modify the chip, The physical modality, chemical composition, and protein response of the biochip were evaluated after the modifications, The results show that the modifications were effective, and the 2,4-D was successfully immobilized. Low non-specific adsorption was achieved at the diamino-poly（ethylene glycol） modified surface with strong binding of the 2,4-D antibody due to the aminodextran modified chip. All three methods can be used to preparation of biochips for optical immunoanalytical equipment.