荣昌猪白细胞介素-8基因cDNA的克隆及序列分析

Cloning and Sequencing of Interleukin-8 cDNA of Rongchang Pig

从体外ConA刺激20 h的荣昌猪外周血淋巴细胞中提取总RNA,应用RT-PCR技术扩增,pMD18-T载体连接,常规转化后,进行酶切及序列测定,并进行鉴定。结果显示:猪IL-8基因与人、猕猴、牛、绵羊和家犬基因序列的同源性分别为81.3%、80.4%、88.9%、88.2%和85.3%。

Total RNA was extracted from peripheral blood lymphocytes of Rongchang pig. By using RT-PCR, subcloning into pMD18-T vector, it was then detected by digesting and sequencing. Result showed that Rongchang pig gene sequence was 81.3 %, 80.4 %, 88.9 %, 88.2 % and 85.3 % homology to those of human, macaque, cattle, sheep and domestic dog, respectively.