异丙酚对大鼠肾缺血再灌注损伤肾组织病理变化的影响

Effect of propofol on renal histopathological changes during acute renal ischemia/reperfusion injury in rats

目的:探讨异丙酚对大鼠肾缺血再灌注损伤的影响。方法:雄性SD大鼠50只,随机分为对照组(C),肾缺血再灌注组(Ⅰ),异丙酚预先给药组(P1)、即时给药组(P2)及延迟给药组(P3)。P1、P2、P3组分别于阻断前1h、阻断同时及再灌注同时缓注异丙酚30mg/(kg.h)3h、4h、4.75h,24h处死大鼠。取肾组织作HE染色及测定丙二醛(MDA),超氧化物歧化酶(SOD)和Ca2+的变化。结果:P1和P2组与I组相比明显改善肾组织损伤,P3组无变化。P1和P2组与Ⅰ组比较MDA和Ca2+水平显著降低,SOD活性增强(P<0.01或0.05),P3组无此作用。结论:异丙酚预先和即时给药明显改善肾损伤,延迟给药无作用。

Objective：To investigate the effects of propofol on renal histopathological changes during acute renal ischemia/reperfusion injury in rats. Methods：A total of 50 SD rats were randomly and equally divided into sham operation group （group C） , renal ischemia/ reperfusion group （ group Ⅰ）, propofol pretreatment group （ group P1 ） , propofol simultaneous treatment group （ group P2 ）, and propofol post-treatment group （ group P3 ）. The rats in groups P1 , P2, and P3 were infused with 30 mg· kg ^- 1. h^ -1 of propofol 1 hour before the occlusion of renal artery for 3 hours, simultaneously with the occlusion of renal artery for 4 hours, simultaneously with the reperfusion for 4.75 hours, respectively. The rats were killed 24 hours later. The renal tissues were observed after HE staining, and the changes in the levels of malondialdehyde （MDA） , superoxide dismutase （SOD） , and Ca^2＋ were measured. Results： Compared with group Ⅰ, in groups P1 and P2, the renal histopathological changes were less severe, the levels of MDA and Ca^2 ＋ significantly decreased, and the activity of SOD significantly increased（ P 〈 0.01 orP 〈 0.05 ）. No such effects were found in group P3. Conclusion： The pretreatment or simultaneous treatment with propofol can ameliorate renal injuries after ischemia/repeffusion, but post-treatment has not such protective effect.