IVF-20培养液用于大鼠体外受精的实验研究

Application of IVF-20 Medium in Studies on In Vitro Fertilization in Rats

目的探讨人用体外受精液用于实验大鼠体外受精的可行性,为实验大鼠的胚胎保种提供参考。方法选用人体外受精IVF-20培养液作为大鼠精子获能和受精培养液,对SD、Wistar、GK、F344等四种不同品系的实验大鼠进行体外受精;用mR1ECM培养液对体外受精所得胚胎进行体外培养实验。同时,将所得2-细胞胚胎移植给经假孕处理的受体鼠。结果四个品系大鼠体外受精后的卵裂率分别可达83.2%、72.6%、87.8%和71.6%。体外受精卵裂胚能够在体外进一步发育,SD大鼠囊胚发育率为16.7%,与体内受精胚胎在体外培养的囊胚发育率(24.5%)差异不显著。80枚2-细胞胚胎移植给2只受体鼠后均妊娠成功,产下正常仔鼠10只,产仔率12.5%。结论用于人体外受精的IVF-20培养液同样适合于实验大鼠精子的体外获能和受精,可以在多种品系获得较高的卵裂率,所得卵裂胚能够在体外发育到囊胚,并且所得卵裂胚在移植给受体鼠后能够产下正常仔鼠。

Objective To explore the possibility of use of IVF-20 medium in rat in vitro fertilization （IVF） experiments, and provide a practical reference for embryo conservation. Methods Using IVF-20 as capacitafion and fertilization medium, four strains of rats, including SD,Wistar, GK and F344 were chosen for the IVF experiment. The resulting IVF embryos were cultured in vitro with mRIEMC medium. Besides, some of 2-cell embryos were transferred into the oviducts of recipients after induction of pseudopragnancy. Results The cleavage rates of SD, Wistar, GK and F344 rats were 83.2%, 72.6%, 87.8% and 71.6%, respectively. The blastocyst development rate in SD rat was 16.7%, lower but with no significant difference than that （24.5%） of embryos produced in vivo. Both recipients transferred with embryos from IVF established pregnancy, and delivered 10 pups （12.5%）. Conelusion IVF-20 medium used in human in vitro fertilization is also suitable for in vitro capacitation and fertilization in rats, resulting in good cleavage rate, as well as blastocyst development rate, and the 2-cell embryos from IVF can fulfill the complete development after transplantation and give birth to normal pups.