固精丹对庆大霉素急性肾损伤的防治作用及机制研究

Effect and mechanism of Gujingdan on acute renal injury induced by gentamicin

目的:研究中药复方固精丹(黄芪、益智仁、丹参、淫羊藿、芡实等)对庆大霉素致大鼠急性肾损伤的保护作用及作用机制。方法:wistar大鼠90只随机分成空白组、模型组、预防组和治疗组。治疗组和预防组又分为固精丹高、中、低(4g/kg、2g/kg、1g/kg)剂量组和阳性对照组(肾康宁2g/kg)。预防组提前一周灌胃给药,一周后,模型组、预防组和治疗组均腹腔注射庆大霉素(100mg/kg),连续7d;预防组继续灌胃给药一周;治疗组于注射庆大霉素的同时,分别灌胃固精丹和肾康宁,连续灌胃7d。在末次给药后4h,接24h尿测N-乙酰-β氨基葡萄糖苷酶(NAG);测血肌酐(SCR)、尿素氮(BUN);取肾脏作光镜和电镜检查;测肾匀浆SOD、MDA及ATP酶活性。结果:与模型组相比,固精丹能明显降低NAG、SCR和BUN的含量,明显提高SOD、ATP酶的活性,降低MDA水平;组织学检查:固精丹高、中剂量组肾小管上皮细胞染色、形态接近空白对照组,明显好于模型组和阳性对照组。结论:固精丹对庆大霉素引起的急性肾损伤具有保护作用;其作用机制可能与固精丹具有抗氧化功能有关。

AIM： To study the protective effect and mechanism of Gujingdan （Radix Astragali, Fructus Alpiniae oxyphyllae, Radix et Rhizoma Salviae miltiorrhizae, Herba Epimedii, Semen Euryales, etc. ） on the acute renal injury caused by Gentamicin in rats. METHODS： 90 rats were randomly divided into normal control group, pathologic model group, preventive group which included high, middle and low dosages of Gujingdan （4 g/kg, 2 g/kg, 1 g/kg） and positive control group which was treated with Shenkangning （2 g/kg） and treated group which included the same groups as the preventive group. The preventive kangning for two weeks and ahead one week. On the seventh groups were respectively given Gujingdan and Shenday, except the normal group the others were continuously injected gentamicin by abdominal cavity （100 mg/kg/day） seven days. At the same time, the treated group were given Gujingdan and Shenkangning by ig for seven days. When the experiment was over, the content of β-N-acetyglocosamidase （NAG）, serum creatinine （SCR） and blood urea nitrogen（BUN） were examined, and the renal histological changes were observed. RESULTS： NAG, BUN, SCR of the groups which were treated with Gujingdan were remarkly lower than that of the pathologic model group; SOD, ATP were higher and MDA was lower than that of the model group. Histological changes showed that renal tubular epithelial cells of the groups treated with Gujingdan were similar to the changes of normal group and much better than that of positive group and pathologic model group. CONCLUSION： Gujingdan has the protective effect on acute renal injury caused by Gentamicin. The mechanism may be that Gujingdan has the function of inhibiting oxidation, protecting and stabilizing cell membrane.