板蓝根挥发油成分的GC/MS分析

GC/MS Analysis of Volatile Constituents of Radix Isatidis

目的:建立以气相色谱-质谱联用技术(GC/MS)分析鉴定板蓝根挥发油成分的方法。方法:采用水蒸气蒸馏法从板蓝根中提取挥发油,色谱柱为OV-1柱,流速为1.0mL.min-1,质谱采用EI离子源;通过NIS107数据系统分析鉴定板蓝根挥发油成分。结果:共分离得到22个色谱峰,鉴定出19个化合物,占总量的90.51%,其中含量最高的是十六酸,约占总量的38.52%。结论:本试验为板蓝根的综合利用提供了初步的试验数据。

OBJECTIVE： To establish a GC-MS method to identify the volatile constituents in Radix Isatidis. METHODS： The method of wet distillation was applied to extracting volatile oil from Radix Isatidis, the chromatographic column was OV - 1, and the flow rate was 1.0mL·min^-1, EI ion source was used by MS. The volatile constituents in Radix Isatidis were identified and analyzed by NIS107 system. RESULTS： 22 chromatographic peaks were separated, 19 chemical compounds were identified, representing 90.51% of total amount, hexadecanoic acid had highest content, accounting for 38.52% of total amount. CONCLUSION： This experiment has provided initial usable data for comprehensive utilization of Radix Isatidis.