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用Chelex-100方法提取肋软骨DNA 105例 被引量:3

The DNA Extraction of Costal Cartilage in 105 Cases by Cheles-100
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摘要 目的研究尸体所处环境、死亡时间的条件下,用Chelex-100方法提取肋软骨DNA.方法对案件中不同条件腐败尸体,采用Chelex-100方法提取肋软骨DNA,进行PCR扩增及STR分型.结果提取肋软骨均可获得STR分型.结论肋软骨是法医检案中的一类具有实用价值的检材. Objective To investigate the DNA extraction of costal cartilage by Chelex-100. Method Decayed body's costal cartilages were selected and its DNA were extracted.PCR amplification and STR typing followed. Result Costal cartilage samples could offer satisfactory results.Conclusion Costal cartilage is a useful sample for forensic case.
出处 《昆明医学院学报》 2007年第02B期64-66,共3页 Journal of Kunming Medical College
关键词 肋软骨 Chelex-100方法 DNA提取 STR Costal cartilage Chelex-100 DNA Extraction STR
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