C57BL/6小鼠系膜增生型肾小球肾炎的复制

Replication of Mesangioproliferative Glomerulonephritis in C57BL/6 Mice

目的探索一种复制小鼠系膜增生型肾小球肾炎动物模型的方法。方法80只C57BL/6小鼠随机分为4组(每组20只):A-正常对照组;B-竹叶青蛇毒2 mg/kg注射组;C-竹叶青蛇毒4 mg/kg注射组;D-竹叶青蛇毒6mg/kg注射组。按照分组要求计算B、C、D 3组小鼠中每只小鼠需注射的竹叶青蛇毒的总量,配制3种不同浓度的竹叶青蛇毒液,B、C、D 3组小鼠分别尾静脉注射不同浓度的竹叶青蛇毒液0.2 mL,A组小鼠尾静脉注射生理盐水0.2 mL以进行对照。结果竹叶青蛇毒注射后的小鼠均出现中毒性症状。C、D组小鼠因蛇毒注射剂量较大,死亡率高达50%,且集中于前3 d内死亡,3 d后中毒小鼠的活动基本如常。B组与正常对照组小鼠无死亡。中毒小鼠的肾功能与正常对照组相较无明显差别,但均出现了肾小球系膜细胞增生,系膜基质增多,肾小球及间质内炎性细胞浸润等病理学改变。结论竹叶青蛇毒尾静脉注射是一种复制小鼠系膜增生型肾小球肾炎动物模型的较好的方法。

Objective To set up a method to replicate mesangioproliferative glomerulonephritis in C57BL/6 mice. Methods Eighty C57BL/6 mice were randomly divided into four groups （n = 20, each group）： group A —normal control; group B — with injection of Habu-snake venom in a dose of 2 mg/kg; group C — with 4 mg/kg Habu-snake venom; group D — with 6 mg/kg Habu-snake venom. The Habu-snake venom was prepared into a volume of 0.2 mL, according to the required dose in groups B, C, D, correspondingly. The venom was injected into the tail vein of mice in groups B, C and D, and 0.2 ml of saline was injected into the tail vein of mice in group A. Results All mice exhibited toxic symptom after Habu-snake venom injection. Mortality of mice in groups C and D reached nearly up to 50% because of the higher dose of Habu-snake venom, and all the mice were died within the first three days after venom injection. All sur~＇iving mice returned gradually to normal activities at 3 days after injection. No mice died in group B and control group. Pathological examination revealed proliferation of mesangial cells, increased amount of mesangial matrix, and inflammator）- cell infiltration in the glomeruli and interstitium in toxin-treated mice, though the renal function showed on significant difference in comparison with that of control mice. Conclusion Habu-snake venom injection via tail vein is a good method to replicate mesangioproliferative glomerulonephritis animal model in C57BL/6 mice.