RP—HPLC测定N—苯基—2,6—二氯苯胺合成反应的终点

The end-point Determination of N-Phenyl-2,6-dichloroani line Synthesis Reaction By HPLC

目的：建立高效液相色谱法测定N—苯基-2,6-二氯苯胺合成反应的终点。方法：采用Shim-PackCLS-ODS色谱柱（15cm×6mm,5μm）;甲醇—水（80：20）为流动相;紫外检测波长254nm。结果：N—苯基—2,6-二氯苯胺与2,6-二氯苯胺在3.22～9.67μg范围内呈良好的线性关系。平均回收率为100.6%,RSD二1.8%。结论：方法简单可行,迅速准确。

Objective： To establish a method for The end-point Determmation of N-Phenyl-2,6- dichloroaniline Synthesis Reaction By HPLC. Methods ： A Shim-Pack CLS-ODS column（15cm×6mm）, 5μm） was used; the mobile phase consisted of methanol-water（80： 20）. The detcting wavelength was at 254nm. Result： The liner range of N-Phenyl-2, 6-dichloroaniline and 2,6-dichloroaniline was within 3.22～9.67μg. The average recovery was 100. 6% RSD was 1.8% （n= 5）, Conclusion： The mothed simple, rapid and accurate.