顶空毛细管气相色谱法测定重组人白细胞介素-2中的残留乙腈

Determination of acetonitrile residual in recombinant human interleukin-2 by head-space capillary gas chromatography

建立重组人白细胞介素-2中乙腈残留量的顶空气相色谱测定方法.采用聚乙二醇-20000石英毛细管柱（30 m×0.25 mm×0.25μm）,高纯氮为载气,顶空进样,氢火焰离子化检测器（FID）检测.乙腈在1.014-5.070μg/mL的质量浓度范围内呈良好的线性关系（相关系数r为0.997 3）,平均回收率为97.2%（相对标准偏差RSD为2.8%）,乙腈的最小检测限为0.6μg/mL.所用方法简便、灵敏度高、重复性好,可用于重组人白细胞介素-2中乙腈残留量的测定.

To establish a method for the determination of acetonirrile residue in Recombinant Human Interleukin-2 ,the head-space GC method was used with PEG-20000 Quartz Capillary column （30 m× 0. 25 mm × 0. 25 μm） , high pure N2 as carrier gas and FID detector . Acetonirrile showed good linearity in the range of 1. 014 5. 070 μg/mL in sample solution （ r = 0. 997 3 ）. The average recovery rate was 97.2 % （ RSD = 2. 8% ）. The minimum detective limit of acetonirrile was 0. 6 μg/mL. This method is sensitive, repeatab suitable for determining acetonitrile residual of recombinant human interleukin-2. le and easy to operate and is suitable for determining acetonitrile residual of recombinant human interleukin-2.