血小板源性生长因子-BB凝胶促进糖尿病大鼠创面愈合的实验研究

Research of Platelet-derived Growth Factor-BB Gel on the Wound Healing of Diabetic Rats

目的:观察血小板源性生长因子(PDGF)-BB凝胶对糖尿病Wistar大鼠全层皮肤缺损创面修复的作用。方法:用直径18 mm的环钻在12只雄性糖尿病Wistar大鼠脊柱两侧各压出1个圆形印迹,外科手术剪沿压痕剪去全层皮肤;将大鼠随机分为空白对照组,凝胶基质对照组和PDGF-BB凝胶治疗组,后两组给药1次/d,至术后14 d。分别测量术后不同时间点3组的血糖、体重、创面上皮化面积、创面收缩百分率、创面闭合百分率,并在术后14 d行组织学观察。结果:术后不同时间点3组血糖、体重值比无统计学意义(P>0.05)。术后7、14 d,PDGF-BB凝胶治疗组与2个对照组比,创面收缩较少(P<0.05),新生上皮面积较多(P<0.05)。术后7 d,PDGF-BB凝胶治疗组与2个对照组比,创面闭合百分率较高(P<0.05)。术后14 d苏木精-伊红染色见PDGF-BB凝胶治疗组新生上皮比2个对照组多,新生真皮明显增厚,且大多数创面被新生上皮覆盖。结论:PDGF-BB凝胶能促进糖尿病大鼠的创面愈合,提高其质量,并有延迟创面收缩的作用。

Objective： To explore the effects of platelet-derived growth factor（PDGF）-BB gel on the wound healing of diabetic rats. Methods： Twelve diabetic rats were randomly divided into blank control group, vehicle control group and PDGF-BB gel treatment group, with four rats in each group. A 1.8 centimeter-diameter full-thickness wound was made on each side of the diabetic rat mid-back. The treatment was applied to each wound once daily for 14 consecutive days after wounding. The re-epithelialization area was measured by planimetry, the percentage of wound closure and the percentage of wound contraction were calculated, and the histology was assessed by hematoxylin-eosin staining. Results： There was no significant difference in blood glucose and body weight among three groups at different time points after wounding （P 〉 0.05）. The percentage of wound contraction was decreased and the re-epithelialization area was enhanced in the PDGF-BB gel treatment group, compared with those in the blank control group and vehicle control group at day 7 and 14 after wounding（P 〈 0.05）. The percentage of wound closure was higher in the PDGF-BB gel treatment group than that in both blank control group and vehicle control group（P〈 0.05）. The epidermis and dermis were thicker in the PDGF-BB gel treatment group than those in the blank control group and vehicle control group at day 14 after wounding by hema- toxylin-eosin staining. Conclusion： PDGF-BB treatment can improve healing quality. PDGF-BB is an important future clinical tool, particularly for stimulating soft tissue repair in patients with an impaired capacity for wound healing.