间日疟传播阻断疫苗Pvs25和Pvs28重组蛋白免疫活性的实验观察

Observations of immunocompetence on Plasmodium vivax transmission-blocking vaccine Pvs25 and Pvs28 recombinant proteins

目的观察间日疟传播阻断疫苗候选抗原Pvs25和Pvs28重组蛋白免疫小鼠后抗体产生情况,从而探讨此两种候选抗原的免疫活性。方法用酵母菌表达的Pvs25和Pvs28重组蛋白皮下免疫DBA/2小鼠;采用ELISA方法,分别对各组小鼠免疫前后不同时间点血清中特异性IgG及其亚类IgG1、IgG2a的水平进行动态检测。结果初次免疫后第28dIgG及其亚类IgG1、IgG2a均出现有意义的升高(P<0.01),至第112dIgG及其亚类IgG1一直持续于较高的水平(P<0.01);而IgG2a水平则于初次免疫后第42d开始下降,但至112d仍高于免疫前及对照组(P<0.05)。结论Pvs25和Pvs28重组蛋白免疫DBA/2小鼠后可产生较高水平的特异性IgG抗体,并以IgG1亚类为主。

To observe the levels of antibodies induced by Pvs25 and Pvs28 immunization of mice and investigate the immunocompetence of the two recombinant proteins. The DBA/2 mice were subcutaneouly injected with rPvs25 or rPvs28 expressed in yeast emulsified in Freund＇s adjuvant. The level of IgG and its subclasses IgG1, IgG2a in serum obtained from each group mice at different times before or after vaccination were assayed by ELISA kits. The level of IgG, IgG1 and IgG2a increased on the day 28 after first vaccination（P 〈0.01）, after this point the level of IgG and IgG1 were persisting on a very high level until day 112 and there was no evidence to decrease; although the level of IgG2a decresed rapidly after this peak ,its level of the recombinant protein group was higher than that of the control group till the 112 days after first vaccination （P〈0. 05）. The rPvs25 and rPvs28 can induce the high level IgG,and the predominant subclass is IgG1. From these results, we conclude that the two recombinant proteins possess the ideal immunocompetence.