^(60)Coγ辐照对草莓花药愈伤组织诱导和分化的影响

EFFECT OF ^(60)Co γ-RAYS IRRADIATION PRETREATMENT ON CALLUS INDUCTIVITY AND DIFFERENTIATION OF STRAWBERRY ANTHER

研究了不同剂量辐照处理花粉发育处于单核靠边期花蕾对草莓花药愈伤组织诱导率和绿苗分化率的影响。结果表明,辐照处理可以明显提高愈伤组织诱导率和绿苗分化率,以辐照剂量20Gy的效果最好,其中章姬品种的愈伤组织诱导率提高39.09%,丰香提高35.68%;在同一种诱导培养基上,经辐照处理产生的愈伤组织,章姬的绿苗分化率与对照相比,提高6.67%~6.72%,丰香提高了4.73%~6.45%。

Strawberry buds, in which a majority of pollen had single nuclear keeping to the side, were pretreated by 60^Coγ- rays irradiation at different doses. The irradiation effect on the callus inductivity and plant differentiation rate of strawberry anther were studied. The results indicated that the pretreatment by 60^Coγ-rays could increase the anther callus inductivity and plant differentiation rate significantly, and 20Gy was the best dose. The callus inductivity of Akihime and Toyonoka increased by 39.09% and 35.68%, respectively, compared with the no-pretreatment materials. On the same media, the plant differentiation rate of Akihime and Toyonoka were also increased by 6.67 % - 6.72 % and 4.73 % - 6.45 %, respectively, compared with the two varieties of no-pretreatment.