PML-RAR_α融合基因检测方法学的建立

Establishment of a method for the detection of PML-RAR α fusion gene

应用筑巢式逆转录聚合酶链式反应（ＲＴ－ＰＣＲ）方法检测２３例急性早幼粒白血病（ＡＰＬ），早幼粒白血病基因ＰＭＬ维甲酸受体α融合基因（ＰＭＬ－ＲＡＲα），肯定了２例与急非淋白血病Ｍ２不易鉴别的不典型ＡＰＬ。１５例缓解在２年以内ＡＰＬ此融合基因均阳性，其中Ｌ型８例，Ｓ型７例，Ｌ型中尚发现一个变异型。８例缓解在３年以上ＡＰＬ，此融合基因４例阳性，其中Ｌ型３例，Ｓ型１例，Ｌ型１例已有复发倾向，而缓解超过４年ＡＰＬ此融合基因均为阴性。

A total of 23 patients with acute promyelocytic leukemia (APL) were examined for promyelocytic leukemia-retinoic acid receptor alpha (PML-RAR α) fusion gene with reverse transcriptase/polymerase chain reaction assay.The PML-RAR α fusion gene was positive in 2 cases of atypical APL which were difficult in differentiation from acute nonlymphoblast leukemia M 2 type.Fifteen cases in this series had been in complete remission (CR) state for 2 years,whose PML-RAR αfusion gene was all positive ,8 cases being expressed as long type including 1 case of abnormal long type and 7 cases as short type.Another 8 cases had been in CR state for 3￣4 years,whose PML-RAR α fusion gene was postive in 4 cases ,3 in 4 cases being expressed as long type,1 in 4 case as short type.One case expressed as long type tended to relapse.On the contrary,the PML-RAR α fusion gene was all negative in the patients who had been in CR state for over 4 years.