血清对氧磷酯酶-1活性及其基因多态性与动脉粥样硬化性血栓性脑梗死关系的研究

Study the relationship between the serum paraoxonase-1 activity and its gene polymorphism and atherosclerotic thrombotic cerebral infarction

目的研究中国汉族人血清对氧磷酯酶-1(PON1)活性及PON1Q/R192基因多态性与动脉粥样硬化性血栓性脑梗死(ATCI)的关系。方法采用紫外线分光光度法和聚合酶链式反应-限制性片段长度多态性(PCR-RELP)方法,检测48例ATCI患者和55名相匹配的健康人血清PON1活性及PON1Q/R192基因多态性。结果ATCI组血清PON1活性为(74.41±18.85)U/ml,明显低于健康对照组[(113.65±26.64)U/ml](P<0.05)。ATCI组与健康对照组等位基因频率分别为Q:37%及43%;R:63%及57%,两组间等位基因频率及3种基因型分布差异无统计学意义。结论血清PON1活性降低是ATCI的危险因素之一;PON1Q/R192基因多态性与中国汉族人ATCI的发生无相关性。

Objective To study the relationship between the serum paraoxonase-1 （ PON1 ） activity, PON1Q/ R192 polymorphism and atherosclerotic thrombotic cerebral infarction （ATCI） in Chinese Han people. Methods In 48 cases with ATCI and 55 normal controls （NC group ）, serum PON1 activity was assayed by infrared spectrophotometer;the PONIQ/R192 polymorphism was determined by polymerase chain reaction and restriction fragment length polymorphism. Results Serum PON1 activity in the ATCI group was significantly lower than that in NC group[（74.41± 18.85） U/ml,（113.65±26.64） U/ml, P〈0.05]. In the groups ATCI and NC,allele frequencies were Q： 37% , 43% ; R： 63%, 57% respectively, there was no significant difference between them. Distributional difference of three genetypes in two groups were also no significant difference. Conclusions Low serum PON1 activity is one of the risk factors of ATCI. There is no significant relationship between PON1Q/R192 polymorphism and ATCI in Chinese Han people.