脂肪型脂肪酸结合蛋白基因多态性与鸡肉品质性状的关系研究

Study of Polymorphysisms in Adipocyte Fatty Acid Binding Protein Gene and Their Relationship with Fatness Related Traits in Chick

采用PCR-SSCP技术分析了A-FABP基因在北京油鸡、矮脚鸡、泰和乌骨鸡、崇仁麻鸡、鹿苑鸡、霞烟鸡等6个地方品种鸡和AA肉鸡中的多态性,发现的3个多态位点的基因型频率和等位基因频率在不同品种之间存在显著差异。测序分析表明:多态位点的产生分别是由碱基C→T、G→A以及C→T的变异引起的。在北京油鸡、矮脚鸡群体中对各多态位点不同基因型与体重、肌内脂肪、腹脂率等脂肪相关性状进行关联分析,结果显示不同基因型间在部分肉品质相关性状上差异显著。

PCR-SSCP was used to detect polymorphic sites in chick adipocyte fatty acid binding protein gene （A-FABP）. Six Chinese local breeds, Beijing Fatty chick, Dwarf chick, Taihe Silky Fowl chick, Chongrenma chick, Xiayan chick, Luyuan chick and an imported breed, Arbor Acre broil, were used as tested populations. Three PCR-SSCP loci were detected. The frequencies of genotype and allele were significantly different in different populations. Sequence analysis revealed that C→T, G→A, and C→T transitions were responsible for the polymorphisms. Some traits related fatness such as body weight, content of intramuscular fat （IMF） and percentage of abdominal fat weight to body weight （AFP） were measured in Beijing Fatty chick and Dwarf chick. We found that meat quality traits were significantly related to different genotypes in the populations tested.