鸡胚精原干细胞定向诱导分化为成骨细胞的初探

The Preliminary Study on Differentiation of Chicken Spermatogonial Stem Cells to Osteoblasts in vitro

研究体外定向诱导鸡胚精原干细胞(Spermatogonial stem cells,SSCs)向成骨细胞分化的能力。采用地塞米松+β-磷酸甘油钠+维生素C组成的诱导液对鸡胚精原干细胞进行诱导,利用Von Kossa’s法、改良的钙钴法及免疫组化I型胶原(CollagenⅠ)进行成骨细胞特性鉴定。结果表明:诱导后成骨细胞形成率达到82%,诱导后的细胞经Von Kossa’s染色可见细胞间布满黑色颗粒,提示有矿化基质沉积;改良钙钴法碱性磷酸酶染色胞浆呈深棕色或深黑色,免疫组化法染色细胞呈阳性。因此可以得出:鸡精原干细胞(SSCs)具有向成骨细胞分化的能力。

Investigate the capability of spermatogonial stem cells（SSCs） differentiating to osteoblasts under different experimental conditions in vitro. In this study, the osteoblasts were induced from SSCs by desamethason, glycerol 2-phosphate disodium salt hydrate and vitamin C and were examined with Von Kossa＇s stain, cytochemistry stain and immunohistochemistry stain （Collagen Ⅰ ）. The results indicated that the osteoblasts formed Von Kossa stain positive nodules, dark granules were found in the cell, suggesting the deposition of mineralized materials; the osteoblasts showed brown or deep dark by alkaline phosphatasepositive stain and showed Collagen Ⅰ positive with immunohistochemistry stain. And so, we may know that the Spermatogonial stem cells（SSCs） have the capability to be differentiated to osteoblasts in vitro.