强骨饮对去势大鼠股骨颈骨形态计量学的影响

Effect of Chinese herbal medicine (Qiangguyin) on histomorphology of femur neck in ovariectomized rats

目的探讨中药强骨饮对去势大鼠骨形态计量学的影响。方法选择雌性SD大鼠60只,随机分为强骨饮组(治疗组)、仙灵骨葆组(对照组)和空白对照组,切除大鼠卵巢2月后,给予中药强骨饮灌胃治疗,并与仙灵骨葆组和空白对照组对照治疗,后分别于3月、4.5月、6月取股骨颈包埋切片,全自动图像分析及骨形态计量学软件处理,观察中药对骨形态计量学参数的影响。结果卵巢切除后大鼠骨小梁面积百分数(%Tb.Ar)下降,骨小梁数量(Tb.N)下降,骨小梁宽度(Tb.Th)下降,经强骨饮治疗3月、4.5月、6月后,与空白对照组比较,Tb.Th差异具有显著性(P<0.01)、与仙灵骨葆组比较,Tb.N差异具有显著性(P<0.05)。结论中药强骨饮具有增加骨量,提高骨质量的作用,说明中药强骨饮对骨质疏松有明显的治疗作用。

Objective To explore the histomorphology effect of Qiangguyin on ovariectomized rats. Methods Sixty female SD rats were divided randomly into three groups： Qiangguyin group（ treatment group）.Xianlinggubao group （contrast group） and control group. All rats were ovariectomized, the Qiangguyin group.Xianlinggubao group and control group were given Qiangguyin .Xianlinggubao and distilled water after two weeks respectively. And 3,4. 5,6 months after the treatment, the femur neck were cut and stained with Massen Golden Trichrome for bone histomorphometric analysis. Results The Tb. Ar.Tb.N.Tb.Th of ovariectomized rats were decreased. Compared with Tb.Th of control group （P 〈 0.01） and rib. N of Xianlinggubao group（ P 〈 0.05）, the differences were observed. Conclusions Chinese herbal medicine （Qiangguyin） can increase the bone quantity and quality. Qiangguyin is effective in treatment of osteoporosis.