镇痛灵对糖尿病并发症的防治作用

Precaution and treatment effects on the complications of diabetes mellitus of super painkiller

目的:研究镇痛灵(SP)对Wistar糖尿病大鼠生殖功能的影响机制。方法:3～5月龄♂Wistar鼠75只,随机分为糖尿病组60只,尾静脉注射1.5%四氧嘧啶50mg·kg-1;正常对照组15只,尾静脉注射等体积生理盐水。72h后,以血糖值大于16.67mol·L-1为造模成功。将糖尿病大鼠随机分为4组,模型组和3种不同剂量镇痛灵治疗组。第5周乙醚麻醉下断头取血,分离血清测生化指标。取一侧睾丸组织,制备病理切片,光镜观察;另一侧制成匀浆液,待测组织成份。结果:(1)糖尿病大鼠与正常对照组比较,血清一氧化氮、一氧化氮合酶、血清总抗氧化能力明显降低,差异有极显著性(P<0.01);黄嘌呤氧化酶值升高(P<0.01);(2)中、高剂量治疗组血清一氧化碳、一氧化碳合酶、血清总抗氧化能力值与模型组比较明显升高,差异有显著性(P<0.05);黄嘌呤氧化酶值降低(P<0.05)。低剂量治疗组各指标变化与模型组比较,差异无显著性(P>0.05);(3)糖尿病大鼠睾丸病理改变明显,经中、高剂量镇痛灵治疗后,损害的间质细胞、生精细胞数量和形态恢复正常。结论:镇痛灵对糖尿病并发的生殖功能障碍有防治作用,并在一定剂量范围内呈量效依赖关系。

OBJECHVE To observe and explore the results relation of Super Painkiller effects experimental the male wistar rats and precaution-treatment system of sexual and reproductive malfunction of diabetes. METHODS 75 wistar rats at 4 - 6 months of age were divided into two groups at random： the group of diabetes mellitus were 60 rats, they were injected with Alloxan （50 mg. kg^-1 1.5 % ） ;Control： 15 rats were injected with the same dose of sodium chlorido injection. After 72 hours, we observed the blood glucose change, when blood glucose was higher than 16. 67 mmol·L^-1 ,The model was formed, the rats of diabetes mellitus were divided into 4 groups. The group of diabetes mellitus and the group of diabetes mellitus was treated with super painkiller. After 5 weeks,we took blood glucose. Testicular morphological changes were observed by using regular histology method. Got blood from vein, killed the rats, the other side were produced of micture, made serum out, measured dora. RESULTS （ 1 ） Compared diabetes rats with normal group, serum NO, NOS, T-AOC dropped sharply, the diversity was significantly. XO increasd （P〈0. 01 ）. （2） Compared with the model group , the serum NO , NOS , T-AOC of therepy （middle, hight） group were very high , the diversity was significantly , XO dropped （P〈0. 05）. low dose groUp not significantly with diabetes. （3） The histology ically diabetic rats changed sharply. By treatment of super painkiller the damaged testis sertoli and spermatogenic cells were normal in the rats at diabetes. CONCLUSION It is proved that super painkiller has surely function of descend blood glucose , it has precaution and treatment function to sexual and reproductive malfuction of diabetes rats.