高效液相色谱-蒸发光散射检测法测定穿山龙中薯蓣皂苷的含量

Determination of Dioscin in Rhizoma dioscoreae nipponicae by HPLC-ELSD

目的:利用高效液相色谱-蒸发光散射检测法对穿山龙中薯蓣皂苷进行分离分析,测定穿山龙中薯蓣皂苷的含量。方法:色谱柱YWGC18柱(250mm×4.6mm,10μm),流动相:乙腈-水(55∶45),流速:1.0mL·min-1,载气流速:2.4mL·min-1,雾化温度:90.3℃左右,柱温:室温。结果:薯蓣皂苷的线性范围为80～600mg·L-1(r=0.9998)。平均回收率为99.88%,RSD为1.25%(n=9)。结论:本方法简便、准确、重复性好,可作为中药穿山龙的质量控制方法。

OBJECTIVE The purpose is to separate the Dioscin in Rhizoma dioscoreae nipponicae, and to develop a new ,quantitative analysis method for the determination of dioscin in Rhizoma dioscorae nipponicae by HPLC-ELSD. METHODS Chromatographic： The peak of dioscin has a retetion time at 9.9 min. The chromatographic condition was follows： chromatographic column： 4. 6 mm× 251） mm,peak with ODS （particle size 10μm）; mobile phase： acetonitrile and water （55：45）; mobile phase flow rate：1. 0 mL.min ^-1 ;column temperature was ambient temperature; clear air pressure：5 × 10^5 Pa and drift tube temperature：90. 3 ℃. RESULTS Dioscin was well-separated. The linear calibration curve was obtained in the concentration rang of 80 - 600 mg. L^- 1 （r = 0. 999 8）. The average recovery of dioscin was 99. 88 %, RSD = 1.25 % （n = 9）. CONCLUSION This method is simple, accurate and reproducible. It is also proposed to be used for quality control of Rhizoma dioscorae nipponicae.