血自由基和肿瘤坏死因子的含量与肝炎病毒感染关系的研究

Research of the Free Radical and TNF in the Blood from Human being Infected by Hepatic Virus

目的探讨肝炎病毒感染与自由基及肿瘤坏死因子的关系。方法用ELISA、显色、比色法检测肝炎病毒感染者、肝炎患者血液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、脂质氧化物(LPO)、丙二醛(MDA)、过氧化氢酶(CAT)、肿瘤坏死因子(TNF)的含量。结果肝炎病毒感染者、肝炎患者血中LPO,MDA,TNF含量明显高于对照组(P<0.05,P<0.01);而SOD,GSH-PX,CAT的含量明显低于对照组(P<0.05,P<0.01),与肝炎病毒感染的种类及肝炎发生的程度密切相关。结论肝炎病毒感染者、肝炎患者的SOD,GSH-PX,CAT,LPO,MDA,TNF的含量检测,对了解肝脏损伤及肝炎的防治有一定意义。

Objective To explore the relationship between infection of hepatic virus and free radical and TNF. Methods The contents of SOD,GSH-PX ,LPO,MDA,CAT and TNF in the blood of infected person and patient of hepatitis were detected receptively by using ELISA and colorimetry. Results The contents of LPO,MDA and TNF in the blood of infected person and patient of hepatitis were significantly higher than those in the blood of the control group（P〈0.05,P〈0.01）. However, the contents of SOD, GSH-PX, CAT were significantly lower than those of the control group （P〈0.05, P〈 0.01）. Those were connected tightly with the type of the hepatic virus infected by Human being and the extent to which hepatitis occurs. Conclusion The test of the contents of SOD,GSH-PX,LPO,MDA,CAT and TNF in the blood of infected person and patient of hepatitis are meaningful to learn the function of liver ,damage of the liver and the prevention and treatment of hepatitis.