水通道蛋白-1在牛角膜内皮细胞液体转运中作用研究(英文)

Research of the role of aquaporins-1 in corneal endothelial fluid transport

目的:研究水通道蛋白-1在牛角膜内皮细胞中的表达及在角膜内皮液体转运中的作用。方法:用免疫组织化学方法,检测水通道蛋白-1在培养的牛角膜内皮细胞中的表达,并用水通道蛋白阻滞剂对氯汞苯磺酸酯(pCMBS)处理培养的牛角膜内皮细胞,观察处理前后角膜内皮细胞的渗透性水通透率(Pf)改变。结果:培养的牛角膜内皮细胞中有水通道蛋白-1的表达,主要位于细胞膜上。汞剂处理前后的角膜内皮细胞Pf分别为(0.044±0.005)cm/s(n=15)和(0.017±0.003)cm/s(n=15),其差异有统计学意义(P<0.01)。结论:水通道蛋白-1在牛角膜内皮细胞液体转运中起着重要作用,其表达的改变会导致角膜水肿和功能改变。

AIM： To investigate the expression of aquaporins-1 （AQP-1） in cultured bovine corneal endothelial cells and to explore the role of AQP-1 in corneal endothelial fluid transport. METHODS：The bovine corneal cells were cultured in DMEM containing 200mL/L neonate bovine serum. AQP-1 expression in the bovine corneal endothelial cells was detected with immunohistochemistry method before and after treatment of the cells with aquaporin inhibitor, p-chloromercuribenzene sulfonate. The osmotic water permeability was determined by monitoring volume changes of cultured bovine corneal endothelial cells. RESULTS： AQP-1 expression in the membrane of cultured bovine corneal endothelial cells was revealed by positive staining （in brown color）. The reading of osmotic water permeability of the cultured bovine corneal endothelial cells before treatment with p-chloromercuribenzene sulfonate was 0.044±0.005cm/s, which significantly decreased to 0.017 0.003 cm/s （n= 15） after treatment. CONCLUSION： AQP-1 expressed in the membrane of cultured bovine corneal endothelial cells may play an important role in fluid transport of corneal endothelial cells. Alteration of the AQP-1 expression may cause abnormal corneal function and corneal edema.