AD7c-NTP与胰岛素受体在小鼠海马CA1区的共存关系

Coexistence of AD7c-NTP and Insulin Receptor in CA1 Zone of Hippocampus in Mouse

目的观察Alzheimer相关的神经丝蛋白(Alzheimer-associated neuronal thread protein,AD7c-NTP)在正常小鼠海马CA1区的分布及其与胰岛素受体的共存关系。方法应用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)和蛋白印迹(Western blot)方法观察小鼠脑蛋白提取物中AD7c-NTP的相对分子质量大小和抗体的特异性。应用免疫组织化学方法观察AD7c-NTP在正常小鼠海马CA1区的分布。应用免疫组织化学双重标记技术观察AD7c-NTP与胰岛素受体的共存关系。结果AD7c-NTP免疫反应阳性条带位于相对分子质量约41 000的位置,与文献报道一致;正常小鼠海马CA1区有AD7c-NTP免疫反应阳性细胞分布,细胞膜和细胞质染色并存在AD7c-NTP和胰岛素受体免疫反应双阳性细胞。结论AD7c-NTP在正常小鼠海马CA1区有分布并与胰岛素受体有共存关系,提示AD7c-NTP的功能可能与胰岛素信号通路有关。

Objective To investigate the distribution of Alzheimer-associated neuronal thread protein （AD7c-NTP） and the coexistence of AD7c-NTP and insulin receptor in CA1 zone of hippocampus in mouse. Methods SDS-PAGE and Western blot were applied to assess the molecular weight of AD7c-NTP in the extract of protein in the brain of mouse and the specificity of the antibody of AD7c-NTP. The distribution of AD7c-NTP in CA1 zone of hippocampus in mouse was investigated with immunohistochemical methods. The coexistence of AD7c-NTP and insulin receptor was studied with double immunohistochemical staining. Results A band of about 41 000 was detected with the antibody of AD7c-NTP, which was coincided with the previous report. AD7c-NTP immunoreactive positive cells were observed in CA1 zone of hippocampus in mouse, whose cellular membrane and cytoplasm were stained. AD7c-NTP and insulin receptor were found coexisting in the same cells in CA1 zone of hippocampus. Conclusion AD7c-NTP is found in CA1 zone of hippocampus in mouse, and coexists with insulin receptor. It indicates that AD7c-NTP may have relation to with the insulin signal pathway.