摘要
目的:建立糖基化终末产物(advanced glycation end products,AGEs)的一种检测方法。方法:利用高效液相色谱议(high performance liquid chromatography,HPLC)构建流动注射分析系统,检测体外制备的AGEs。荧光检测的激发波长和发射波长(Ex／Em)为370／440 nm。结果:Ex／Em为370／440 nm时,在原反应液的0．1～0．000 1浓度范围内仍有良好的线性,Y=67 979．98X+4．38,r=0．999 99;平均回收率98．5％,RSD= 3．4％。结论:流动注射分析(flow injection assay,FIA)是较可靠的检测AGEs水平的新方法。
Objective： To establish an analyzing approach for advanced glycation end products formed in vitro. Methods： Flow injection system was assembled using HPLC to detect AGEs formed in vitro. Fluorescence of each sample was determined at excitation/emission wavelengths of 370/440 nm. Results： There was a good liner between AGEs concentration （diluted to 0.1-0.000 1） and peak area （obtained at 440 nm with excitation at 370 nm）. The equation was y = 67 979.98 x ＋ 4.38, r = 0.999 99. The average recovery rate was 98.5%, RSD = 3.4%. Conclusions： AGEs detected by flow injection assay show excellent accuracy and reproducibility. It is a novel method for monitoring AGEs formed in vitro.
出处
《抗感染药学》
2007年第2期66-68,共3页
Anti-infection Pharmacy
