HPLC法快速测定软饮料中苯甲酸、糖精钠、山梨酸的含量

Quick test of contents of benzoic acid,saccharin acid and sorbic acid in soft drinks by using HPLC method

采用反相高效液相色谱法(HPLC)对碳酸饮料、冰红茶、橙汁中的苯甲酸、山梨酸、糖精钠含量进行了测定。建立了快速分析这三种添加剂的方法,最佳色谱条件为:柱温35℃,流动相体系为甲醇与0.02M,乙酸铵液比例20+80。采用外标法定量,得到了良好的线性关系。回收率在90—110%之间,相对标准偏差小于2%。

By using HPLC method,the contents of benzoic acid,saccharin acid and sorbis acid are tested in carbonioc acid drinks,ice black tea,and orange juice. An analytical method is set up to quickly separate the three addictives . Here are the best chromatographic conditions for the separation：The colum temperature is 35℃ ,and the volume ratio of methano and 0. 02M NH4Ac is 204-80. A good linear relationship is obtained by ESTD. The recovery rate is between 90% and 110% ,and the relative standard deviation is smaller than 2%.