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rRNA同源性分析方法在微生物分类中的应用 被引量：1

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摘要 概述了核酸杂交、rRNA寡核苷酸编目、rRNA全序列分析、特征序列、转录间隔区序列分析、扩增rDNA限制性酶切片段分析与变性梯度胶电泳技术等7种涉及rRNA同源性分析的分子生物学技术,在微生物分类中的应用情况及其贡献和各自的优点与局限性。rRNA同源性分析方法作为微生物系统分类的主要依据已经得到了广泛认同。
作者 王慧杰 杨向科 马爱民
机构地区 郑州牧业工程高等专科学校 华中农业大学
出处 《河南农业科学》 CSCD 北大核心 2007年第5期14-17,共4页 Journal of Henan Agricultural Sciences
基金 河南省重点科技攻关项目(0423011200)
关键词 RRNA 微生物分类 鉴定 系统发育
分类号 Q52 [生物学—生物化学]
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参考文献16

  • 1Glazer A N,Nikaido H.微生物生物技术-应用微生物学基础原理[M].陈守文,喻子牛,刘子峰,等译.北京:科学出版社,2002:41-43.
  • 2鲁辛辛,刘向祎,李大为.微生物基因分类鉴定的方法学进展[J].中国实验诊断学,2003,7(3):201-206. 被引量：7
  • 3Nygaard A P,Hall B D.A method for the detection of RNA-DNA complexes[J].Biochem Biophys Res Commun,1962,12:98-104.
  • 4Gillespie D,Spiegelman S.A quantitative assay for DNA-DNA hybrids with DNA immobilized on a membrane[J].Mol Biol,1965,12:829-842.
  • 5Takahashi M,Saito M,Ikeda Y.Species specificity of the ribosomal RNA cistrins in bacteria[J].Biochem Biophys Acata,1967,134:124-133.
  • 6葛泉卿,温孚江.病原微生物的生物大分子指纹图谱分类及分型鉴定技术[J].检验检疫科学,2004,14(1):35-41. 被引量：1
  • 7Woese C R.Bacterial evolution[J].Microbiol Rev,1987,51:221-271.
  • 8Woese C R,Kandler O,Wheelis M L.Towards a natural system of organisms:proposal for the domains Archaea,Bacteria and Eukarya[J].Proc Natl Acad Sci,1990,87:4576-4579.
  • 9Julian R,Marchesi,Takuichi Sato,et al.Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA[J].Applied and Environmental Microbiology,1998,64:795-799.
  • 10洪义国,孙谧,张云波,李勃生.16SrRNA在海洋微生物系统分子分类鉴定及分子检测中的应用[J].海洋水产研究,2002,23(1):58-63. 被引量：61

二级参考文献24

  • 1萨姆布鲁克J 弗里奇F.分子克隆实验指南，第二版[M].北京:科学出版社,1996..
  • 2De-Santis R, Azzi A. Use of denaturing gradient gel electrophoresis for human polyomavirus JC sequence analysis. J Virol Methods, 2000, 85(1-2): 101-108.
  • 3Matousek J, Ptacek J, Dedic P, et al. Analysis of variability of P1 gene region of N strain of potato virus Y using temperature-gradient gel electrophoresis and DNA heteroduplex analysis. Acta Virol, 2000, 44(1): 41-46.
  • 4Harris KA, Teo CG. Diversity of hepatitis C virus quasispecies evaluated by denaturing gradient gel electrophoresis. Clin Diagn Lab Immunol, 2001, 8(1): 62-73.
  • 5Cheung PY, Kinkle BK. Mycobacterium diversity and pyrene mineralization in petroleum-contaminated soils. Appl Environ Microbiol, 2001, 67(5): 2222-2229.
  • 6Cocolin L, Manzano M, Cantoni C, et al. Development of a rapid method for the identification of Lactobacillus spp. isolated from naturally fermented italian sausages using a polymerase chain reaction-temperature gradient gel electrophoresis. Lett Appl Microbiol, 2000, 30(2): 126-129.
  • 7Tannock GW. Analysis of the intestinal microflora using molecular methods. Eur J Clin Nutr, 2002, 56 (Suppl 4): S44- S49.
  • 8Favier CF, Vaughan EE, De-Vos WM, et al. Molecular monitoring of succession of bacterial communities in human neonates. Appl Environ Microbiol, 2002, 68(1): 219-226.
  • 9Nielsen DS, Moller PL, Rosenfeldt V, et al. Case study of the distribution of mucosa-associated Bifidobacterium species, Lactobacillus species, and other lactic acid bacteria in the human colon. Appl Environ Microbiol, 2003, 69(12): 7545-7548.
  • 10Burton JP, Cadieux PA, Reid G. Improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation. Appl Environ Microbiol, 2003, 69(1): 97-101.

共引文献148

同被引文献13

  • 1王利,何军,余冰,黄志清,毛湘冰,郑萍,陈代文.小麦基础饲粮中添加木聚糖酶对生长猪生长性能、血清生化指标及肠道微生物菌群的影响[J].动物营养学报,2012,24(10):1920-1927. 被引量：25
  • 2刘建高,张军,印遇龙,LIU Qiang.抗性淀粉的功能及影响其含量的因素[J].华北农学报,2006,21(B10):1-5. 被引量：13
  • 3张军,李铁军,宾石玉,黄瑞林,张平,印遇龙,刘兆金.不同来源淀粉对断奶仔猪氮代谢的影响[J].华北农学报,2006,21(B10):177-180. 被引量：4
  • 4Yin Y L, Baidoo S K, Boychuk J L,et al. Performance and carcass characteristics of growing pigs and broilers fed di- ets containing micronized barely, ground barely, wheat and maize[ J ]. Journal of the Science of Food and Agricul- ture,2001,81:1487 - 1497.
  • 5Weurding R E,Enfing H,Verstegen M W A. The effect of site of starch digestion on performance of broiler chickens [ J ]. Animal Feed Science and Technology, 2003, 110 : 175 - 184.
  • 6Cummings J H, Englyst H N. Gastrointestinal effects of food carbohydrate [ J ]. American Journal of Clinical Nu- trition, 1995,61:938 - 945.
  • 7Kittelmann S, Janssen P H. Characterization of Rumen ciliate community composition in domestic sheep, deer, and cattle, feeding on varying diets, by means of PCR- DGGE and clone libraries[ J]. FEMS Microbiology Ecol- ogy ,2011,75 ( 3 ) :468 - 481.
  • 8Weurding R E,Veldman A,Veen W A G, et al. Digestion rate in the small intestine of broiler chickens differs a- mong feedstuffs [ J ]. Journal of Nutrition, 2001,131 ( 9 ) : 2329 - 2335.
  • 9Liu W T, Marsh T L, Chen H,et al. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA [ J ]. Applied and Environmental Microbiology, 1997,63 ( 11 ) :4516 - 4522.
  • 10Shyu C, Soule T, Bent S J,et al. MiCA : A web-based tool for the analysis of microbial communities based on termi- nal-restriction fragment length polymorphisms of 16S and 18S rRNA genes [ J ]. Microbial Ecology, 2007,53 ( 4 ) : 562 - 570.

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河南农业科学
2007年 第5期

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