六味地黄丸(浓缩丸)含量测定方法研究

Determination of Liuwei Dihuang Pill(concentrated pill)

目的建立六味地黄丸（浓缩丸）中测定丹皮酚、马钱苷含量的高效液相色谱方法。方法丹皮酚：色谱柱：C18柱（依利特;250 mm×4.6 mm,5μm）,流动相：甲醇-水（6∶4）,流速1.0 ml·min^-1,柱温30℃,检测波长为274 nm。马钱苷：C18柱（依利特;250 mm×4.6 mm,5μm）,乙腈-水（15∶85）,流速1.0 ml.min-1,柱温25℃,检测波长为236 nm。结果丹皮酚在54.1-270.5 ng范围内呈线性关系,r=0.9996;平均加样回收率为99.42%,RSD=1.08%。马钱苷在0.223-4.46μg范围内呈线性关系,r=0.9996;平均加样回收率为99.53%,RSD=2.15%。结论该方法简便,重现性好,可用于六味地黄丸（浓缩丸）中丹皮酚、马钱苷的含量测定。

Objective To establish an HPLC for determination of paeonol and loganin in Liuwei Dihang Pills （ concentrated pills）. Methods Paeonol ：HPLC column was C18-The mobile phase was methanol -water（6： 4） and the flow rate was 1.0 ml·min^-1. The column temperature was at 30℃ and the detection wavelength was at 274 nm. Loganin：HPLC column was C18. The mobile phase was acetonitrile- water （15： 85） and the flow rate was 1.0 ml·min^-1 The column temperature was at 25℃ and the detection wavelength was at 236 nm. Results The linear range was from 54.1 ng to 270.5 ng（r = 0.999 6） for paeonol and from 0.223μg to 4.46 μg（r = 0.999 6） for loganin. Conclusion The method is convenient and reproducible for determination of paeonol and loganin in Liuwei Dihuang Pills（ concentrated pills）