生脉散复方制剂对体外感染柯萨奇B_3病毒的大鼠心肌细胞的影响

Effect of Pulse-activating Powder Combined with the Roast Glycyrrhiza Decoction on Cultured Rat Myocardial Cells Infected with Coxsackie Virus B_3

目的探讨生脉散复方制剂对病毒性心肌炎的影响。方法取新生SD大鼠心室肌培养48 h后接种103TCID50的柯萨奇B3病毒,观察生脉散合炙甘草汤加减液Ⅰ、生脉散合炙甘草汤加减液Ⅱ对感染病毒大鼠心肌细胞的影响。结果生脉散合炙甘草汤加减液Ⅱ明显减轻感染病毒后心肌病变,改善细胞搏动,明显降低心肌细胞释放酶(乳酸脱氢酶、磷酸肌酸激酶)活性,减轻超微结构病变。结论生脉散合炙甘草汤加减液Ⅱ对感染柯萨奇B3病毒SD大鼠培养心肌细胞具有抗病毒和明显的保护作用。生脉散合炙甘草汤加减液Ⅱ的作用优于不加虎杖的生脉散合炙甘草汤加减液Ⅰ。

Objective To study the anti - viral effect and its mechanism of pulse - activating powder combined with the roast Glycyrrhiza decoction adjusted juice Ⅱ （PGP Ⅱ） on the cultured rat myocardial cells were infected with Coxsackie virus B3. Methotis The beating myocardial cells were obtained from the ventrlcular myocardlum of newborn Sparaque - Dawlrvey rats and incubated for 48 hours and inoculated with 103TCID50 of Coxsackie virus B3 virus, The effect of pulse - activating powder combined with the roast Glycyrrhiza decoction adjusted juiceⅠ , （ PGP Ⅰ） and PGP Ⅱ on the viral mupcarditis was obseeed, Results The PGP Ⅱsignificantly alleviated the pacthologlcal changes of myocardial cells, improved the percent of the beating cell, reduced the activities of lactate dehydrogenase and creatine phosphokinase and alleviated the uhrastructural changes of myocardial cells infected with Coxsackie virus B3 （CVB3 ）. Conclusion The PGP Ⅱhas anti -viral effect and significant protective effect on SD rat myocardial cells infected by Coxsackie virus B3, The effect of PGP Ⅱis better than that of PGP I without Polygonum euspidatum,