α_1-酸性糖蛋白柱拆分西酞普兰对映异构体

Enantiomeric separation of citalopram by HPLC using α_1-acid glycoprotein chiral stationary phase

目的:建立西酞普兰对映异构体的拆分方法。方法:采用α1-酸性糖蛋白(AGP)手性柱固定相,考察了流动相组成、pH值、流速、柱温、有机改性剂对拆分的影响,优化后色谱条件为Chrom Tech CHIRAL-AGP柱(150 mm×4.0 mm,5μm),流动相为50 mmol/L醋酸铵缓冲液(乙酸调节pH至4.3),流速0.8 mL/min,检测波长240 nm,柱温25℃。结果:西酞普兰对映异构体保留时间分别为6.8和8.4 min,能够达到基线分离。结论:缓冲盐浓度和pH值是影响对映体分离的重要因素,同时可以在一定范围内调节流速和柱温来提高柱效,改善分离。

Aim： To develop a chiral separation method for enantiomers of citalopram by HPLC using α1-acid glycoprotein （α1-AGP） chiral stationary phase. Methods：Influences of the pH value of the mobile phase, the kind and concentration of the buffer solution, organic modifiers, the flow rate and the column temperature on the enantiomeric separation of citalopram using α1-AGP chiral stationary phase were investigated. Results： Citalopram enantiomers were separated on an α1-AGP chiral stationary phase column using water as the mobile phase consisting of 50 mmol/L NH4Ac buffer （pH = 4.3） at a flow rate of 0.8 mL/min. Conclusion： The pH value of the mobile phase and the concentration of the buffer solution are very important parameters in the optimization of the enantiomeric separation of the cationic racemates. Higher column efficiency can be achieved with lower flow rate.