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多表位DNA壳聚糖微球疫苗的体液免疫应答 被引量：1

Humoral Immune Response Induced by Polytope DNA Vaccine in Chitosan-based Microspheres
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摘要 目的研究多表位DNA壳聚糖微球疫苗的体液免疫应答。方法制备多表位DNA壳聚糖微球疫苗pcD-NA3.1-HME-3C3d,经鼻腔免疫小鼠,蛋白检测微孔试剂盒检测小鼠特异性IgG抗体水平。结果经免疫的小鼠均能产生针对各表位的特异性IgG抗体,DNA壳聚糖微球疫苗的抗体水平明显高于DNA疫苗。结论壳聚糖微球疫苗投递系统可提高多表位DNA疫苗的免疫应答效果。 Objective To study the humoral immune response induced by polytope DNA vaccine in chitosan-based microsphere. Methods Prepare the constructed DNA vaccines pcDNA3. 1-HME and pcDNA3.1-HME-3C3d into chitosan-based microspheres and immunize mice intranasally. Determine the specific antibody level induced in mice by micropore kit for protein detection. Results The specific IgGs to various epitopes were induced in immunized mice. However, the IgG level induced by chitosan-based DNA microsphere vaccine was significantly higher than that by DNA vaccine. Conclusion The chitosan-based microsphere delivery system enhanced the humoral immune response induced by polytope DNA vaccine.
作者 程玉兴 朱高红 瞿素 兰芸 胡云章 胡凝珠
机构地区 中国医学科学院中国协和医科大学医学生物学研究所疫苗研究室 昆明医学院附一院核医学科
出处 《中国生物制品学杂志》 CAS CSCD 2007年第6期429-431,共3页 Chinese Journal of Biologicals
关键词 多表位DNA疫苗 体液免疫应答 壳聚糖微球 Polytope DNA vaccine Humoral immune response Chitosan-based microsphere
分类号 R392 [医药卫生—免疫学] R979.2 [医药卫生—药品]
  • 相关文献

参考文献11

  • 1Suhrbier A.Multi-epitope DNA vaccines.Immunol Cell Biol,1997,75(4):402-408.
  • 2Ulmer JB,Donnelly JJ,Liu MA.Toward the development of DNA vaccines.Curr Opin Biotechnol,1996,7(6):653-658.
  • 3Dempsey PW,Allison ME,Akkaraju S,et al.C3d of complementas a molecular adjuvant:bridging innate and acquired immunity.Science,1996,271(5247):348-350.
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二级参考文献10

  • 1Suhrbier A. Multi-epitope DNA vaccines. Immunol Cell Biol,1997, 75(4): 402-408.
  • 2Ulmer JB, Donnelly JJ, Liu MA. Toward the development of DNA vaccines. Curt Opin Biotechnol, 1996, 7(6) : 653-658.
  • 3Dempsey PW, Allison ME, Akkaraju S, et al. C3d of complement as a molecular adjuvant: bridging innate and acquired immunity. Science, 1996, 271 (5247) : 348-350.
  • 4Haas KM, Hasegawa M, Steeber DA, et al. Complement receptors CD21/35 link innate and protective immunity during Streptococcus pneumoniae infection by regulating IgG3 antibody responses. Immunity, 2002, 17(6) : 713-723.
  • 5Maeng CY, Ryu CJ, Gripon P, et al. Fine mapping of virus-neutralizing epitopes on hepatitis B virus PreS1. Virology, 2000, 270 (1): 9-16.
  • 6Hirabayashi Y, Fukuda H, Kimura J, et al. Identification of peptides mimicking the antigenicity and immunogenicity of conformational epitopes on Japanese encephalitis virus protein using synthetic peptide libraries. J Virol Methods, 1996, 61(1-2) : 23-36.
  • 7Obeid OE, Partides CD, Howard CR, et al. Protection against morbillivirus-induced encephalitis by immunization with a rationally designed synthetic peptide vaccine containing B- and T-cell epitopes from the fusion protein of measles virus. J Virol, 1995, 69(3) : 1420-1428.
  • 8Martins LP, Lau LL, Asano MS, et al. DNA vaccination against persistent viral infection. J Virol, 1995, 69(4) : 2574-2582.
  • 9Carroll MC. The role of complement and complement receptors in induction and regulation of immunity. Annu Rev Immunol, 1998, 16 : 545-568.
  • 10Haas KM, Toapanta FR, Oliver JA, et al. Cutting edge: C3d functions as a molecular adjuvant in the absence of CD21/35 expression. J Immunol, 2004, 172(10): 5833-5837.

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中国生物制品学杂志
2007年 第6期

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