猪链球菌2型的分离与鉴定

Isolation and Identification of Swine Streptococus suis Serotype 2

目的对从四川省某猪场分离到的5株细菌进行系统鉴定。方法观察菌体的形态、培养特性、动物致病性和生化反应,并采用PCR法鉴定其血清型和毒力因子。结果5株细菌均为猪链球菌2型,均有毒力因子MRP和EF,其中S1株、S2株和S5株对BALB/c小鼠有致病性,另2株无致病性。结论该猪场的疫情是由猪链球菌2型引起的。本研究为进一步预防和控制猪链球菌2型的流行提供了依据。

Objective To identify the 5 strains of swine Streptococus suis isolated from a pig farm in Sichuan Province, China. Methods Observe the morphology,culture characteristics, pathogenicity in animals and biochemical reactions of the 5 strains of swine Streptococus suis ,and identify their serotypes and virulence factors by PCR. Results All the 5 strains were identified as swine Streptococus suis serotype 2 and contained virulence factors MRP and EF ,of which strains S1 ,S2 and S5 showed pathogenicity in BALB/c mice while the other 2 strains showed no pathogenicity. Conclusion The pestilence in the pig farm was caused by swine Streptococus suis serotype 2, which provided a basis for further prevention and control of infection with swine Streptococus suis serotype 2.